Construct: ORF TRCN0000468258
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003783.1_s317c1
- Derived from:
- ccsbBroadEn_14980
- DNA Barcode:
- GCTAACATTCCGTACCTTGTTAGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HNRNPA0 (10949)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468258
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10949 | HNRNPA0 | heterogeneous nuclear ribon... | NM_006805.4 | 61.7% | 61.3% | 559G>A;565_567delCGAinsT;570_915del |
| 2 | mouse | 77134 | Hnrnpa0 | heterogeneous nuclear ribon... | NM_029872.1 | 54.6% | 59.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 633
- ORF length:
- 567
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gaattctcag ttgtgtaagc tgttcatcgg cggcctcaat gtgcagacga 121 gtgagtcggg cctgcgcggc cactttgagg cctttgggac tctgacggac tgcgtggtgg 181 tggtgaatcc ccagaccaag cgctcccgtt gctttggctt cgtgaccTAC TCCAATGTGG 241 AGGAGGCGGA CGCCGCCATG GCCGCCTCGC CCCATGCCGT GGACGGCAAC ACTGTGGAGC 301 TGAAGCGGGC GGTGTCCCGG GAGGATTCGG CGCGGCCCGG TGCCCACGCC AAGGTTAAGA 361 AGCTCTTTGT CGGAGGCCTT AAAGGAGACG TGGCTGAGGG CGACCTGATC GAGCACTTCT 421 CGCAGTTTGG CACCGTGGAA AAGGCCGAGA TTATTGCCGA CAAGCAGTCC GGCAAGAAGC 481 GTGGATTCGG CTTCGTGTAT TTCCAGAATC ACGACGCGGC AGACAAGGCC GCGGTGGTCA 541 AGTTCCATCC GATTCAGGGC CATCGCGTGG AGGTGAAGAA AGCAGTCCCC AAGGAGGATA 601 TCTACTCCGG TGGGGGTGGA GGCAGCTCCT TCTACCCAAC TTTCTTGTAC AAAGTGGTTG 661 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 721 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAGC 781 TAACATTCCG TACCTTGTTA GCACGCGTTA AGTCgacaat caacctctgg attacaaaat 841 ttgtgaaaga tt