Construct: ORF TRCN0000468264
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003062.1_s317c1
- Derived from:
- ccsbBroadEn_12242
- DNA Barcode:
- TGGCTCTGTAATTCAGATGTGTAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RNF126 (55658)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468264
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55658 | RNF126 | ring finger protein 126 | NM_194460.3 | 94.9% | 94.4% | (many diffs) |
| 2 | human | 55658 | RNF126 | ring finger protein 126 | NM_001366018.1 | 86.7% | 86.1% | (many diffs) |
| 3 | human | 376412 | RNF126P1 | ring finger protein 126 pse... | NR_002818.2 | 60.7% | (many diffs) | |
| 4 | human | 55658 | RNF126 | ring finger protein 126 | XR_002958330.1 | 38.4% | (many diffs) | |
| 5 | mouse | 70294 | Rnf126 | ring finger protein 126 | NM_144528.3 | 82.5% | 88.4% | (many diffs) |
| 6 | mouse | 70294 | Rnf126 | ring finger protein 126 | XM_017314081.1 | 75.6% | 69.5% | (many diffs) |
| 7 | mouse | 70294 | Rnf126 | ring finger protein 126 | XM_006514075.3 | 70.2% | 53.9% | (many diffs) |
| 8 | mouse | 70294 | Rnf126 | ring finger protein 126 | XR_001779593.1 | 48.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1044
- ORF length:
- 978
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cgaggcgtcg ccgcatcccg gacggtactt ctgccactgc tgctccgtgg 121 agatcgtccc gcgcctgccg gattatatct gtccaagatg cgagtctggt tttatcgagg 181 agcttccgga agagaccagg agcacagaaa atggttctgc cccctccaca gctcccacag 241 accagagccg gccaccgttg gagcacgtgg accagcacct gttcacgctg ccgcagggct 301 acggacagtt tgctttcggc atcttcgatg acagcttcga gatccccacg ttccctcctg 361 gggcgcaggc tgacgacggc agggaccctg agagccggcg ggagagagac catccgtccc 421 ggcaccggta cggcgcccga cagccccgcg cccgcctcac cacgcggcgg gccaccggcc 481 ggcacgaagg cgtccccacg ctggaaggga tcatccagca gctcgtcaac ggcatcatca 541 cgcccgccac catccccagc ctgggcccct ggggagtcct gcactcaaac cctatggact 601 acgcctgggg ggccaacggc ctggatgcca tcatcacaca gctcctcaat cagtttgaaa 661 acacaggccc cccaccggca gataaagaga aaatccaggc cctccccacc gtccccgtca 721 ctgaggagca cgtaggctcc gggctcgagt gccctgtgtg caaggacgac tacgcgctgg 781 gtgagcgtgt gcggcagctg ccctgcaacc acctgttcca cgacggcTGC ATCGTGCCCT 841 GGCTGGAGCA GCACGACAGC TGCCCCGTCT GCCGAAAAAG CCTCACGGGA CAGAACACGG 901 CCACGAACCC CCCTGGCCTC ACTGGGGTGA GCTTCTCCTC CTCGTCGTCA TCGTCCTCCT 961 CCAGCTCGCC CAGCAACGAG AACGCCACAT GGTCCCCTCT GGGGCGTCCC CAGCCTCCCC 1021 GTCCTCTGTC TAACCTCACC CTCTACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1081 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1141 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT GGCTCTGTAA 1201 TTCAGATGTG TAGACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1261 att