Construct: ORF TRCN0000468272
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010397.1_s317c1
- Derived from:
- ccsbBroadEn_08464
- DNA Barcode:
- TAGGAACGCTTGAGGCACGTACTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- WIPI1 (55062)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468272
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55062 | WIPI1 | WD repeat domain, phosphoin... | NM_017983.7 | 99.9% | 99.7% | 92C>T |
| 2 | human | 55062 | WIPI1 | WD repeat domain, phosphoin... | NM_001320772.1 | 81.6% | 81.6% | 0_1ins246 |
| 3 | human | 55062 | WIPI1 | WD repeat domain, phosphoin... | XM_017024808.1 | 71.3% | 71.3% | 0_1ins384 |
| 4 | human | 55062 | WIPI1 | WD repeat domain, phosphoin... | NR_135471.1 | 65.3% | (many diffs) | |
| 5 | human | 55062 | WIPI1 | WD repeat domain, phosphoin... | NR_135470.1 | 64.5% | (many diffs) | |
| 6 | mouse | 52639 | Wipi1 | WD repeat domain, phosphoin... | NM_145940.2 | 87.5% | 94.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1404
- ORF length:
- 1338
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ggccgaggcc gcggacgctc ccccgggcgg ggttgagtcg gcgctcagct 121 gcttctcttt caaccaggac tgcacatccc tagcaattgg aactaaagcc gggtataagc 181 tgttttctct gagttctgtg gagcagctgg atcaagtcca cggaagcaat gaaatcccgg 241 acgtctacat cgtggagcgc ctcttctcca gcagcctggt ggtggtagtc agtcacacaa 301 aaccacggca gatgaacgtg tatcacttca agaaaggcac agagatctgt aattacagct 361 actccagcaa catcttgtcc ataaggctga accggcaaag gctgctggtt tgcctagaag 421 agtccattta tattcacaac attaaagaca tgaagctgtt gaagaccctc ctggatattc 481 ctgcaaaccc aacaggtcta tgtgctctct ctatcaacca ttccaattct tacctggcct 541 atcctggaag cctgacttca ggggagattg tgctttatga tggaaactcc ctgaaaacag 601 tctgcactat tgctgcccat gagggaacac tagctgccat caccttcaat gcctcaggct 661 ccaaactagc aagtgcgtct gaaaaaggca cagtcatccg ggtgttctct gtccctgatg 721 ggcaaaagct ctatgagttc cggagaggga tgaaaaggta tgtgacaatc agctctctag 781 tgttcagtat ggattcacaa ttcctctgcg cctccagtaa caccgagacg gtacacatct 841 tcaagctgga acaggtcacc aacagtcgac cagaagagcc ttcgacctgg agtggctaca 901 tgggaaagat gtttatggct gctaccaact acctccctac ccaggtgtca gacatgatgc 961 atcaggacag ggcttttgcc actgcacgct tgaacttcTC CGGACAGAGG AACATCTGTA 1021 CCCTCTCAAC GATCCAGAAG TTGCCACGGC TGCTAGTTGC GTCATCCAGT GGACACCTTT 1081 ATATGTACAA TTTGGATCCT CAGGATGGAG GAGAGTGTGT CTTAATCAAA ACCCACAGCT 1141 TGCTTGGCTC AGGAACAACA GAAGAGAATA AAGAAAATGA CCTCAGACCT TCCTTACCTC 1201 AGTCTTATGC AGCGACCGTA GCCAGACCAA GTGCATCTTC AGCCTCCACG GTGCCAGGTT 1261 ATTCTGAGGA CGGCGGGGCG CTGCGAGGAG AAGTTATTCC TGAACATGAG TTTGCGACGG 1321 GACCAGTGTG TCTTGATGAT GAGAATGAGT TTCCTCCTAT AATCTTGTGC CGTGGAAATC 1381 AGAAGGGCAA AACGAAGCAG TCATGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1441 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1501 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT AGGAACGCTT 1561 GAGGCACGTA CTGACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1621 att