Construct: ORF TRCN0000468280
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009213.1_s317c1
- Derived from:
- ccsbBroadEn_13862
- DNA Barcode:
- CCGAGACCGTTCAATCCATCCATT
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GNG7 (2788)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468280
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2788 | GNG7 | G protein subunit gamma 7 | NM_052847.3 | 99.5% | 87.1% | 183delC |
| 2 | human | 2788 | GNG7 | G protein subunit gamma 7 | XM_017026606.1 | 99.5% | 87.1% | 183delC |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 179
- ORF end:
- 383
- ORF length:
- 204
- Sequence:
-
1 GAGACTGAAG TTAGGCCAGC TTGGCACTTG ATGTAATTCT CCTTGGAATT TGCCCTTTTT 61 GAGTTTGGAT CTTGGTTCAT TCTCAAGCCT CAGACAGTGG TTCAAAGTTT TTTTCTTCCA 121 TTTCAGGTGT CGTGAGGCTA GCATCGATTG ATCAACAAGT TTGTACAAAA AAGTTGGCAT 181 GTCAGCCACT AACAACATAG CCCAGGCCCG GAAGCTGGTG GAACAGCTAC GCATAGAAGC 241 CGGGATTGAG CGCATCAAGG TCTCCAAAGC GGCGTCTGAC CTCATGAGCT ACTGTGAGCA 301 ACATGCCCGG AACGACCCCC TGCTGGTCGG AGTCCCTGCC TCGGAGAACC CCTTTAAGGA 361 AAGAAACCTT GTATTATTTT ATACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG 421 CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT 481 GAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACCGA GACCGTTCAA 541 TCCATCCATT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt