Transcript: Human XM_017026606.1

PREDICTED: Homo sapiens G protein subunit gamma 7 (GNG7), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
GNG7 (2788)
Length:
4356
CDS:
347..553

Additional Resources:

NCBI RefSeq record:
XM_017026606.1
NBCI Gene record:
GNG7 (2788)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017026606.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000008811 GTGGAACAGCTACGCATAGAA pLKO.1 386 CDS 100% 5.625 7.875 N GNG7 n/a
2 TRCN0000036815 CTACGCATAGAAGCCGGGATT pLKO.1 395 CDS 100% 4.050 5.670 N GNG7 n/a
3 TRCN0000008809 GCAGGGCATCATTCAGTAATT pLKO.1 626 3UTR 100% 13.200 10.560 N GNG7 n/a
4 TRCN0000036817 CAAAGCGGCGTCTGACCTCAT pLKO.1 433 CDS 100% 1.350 1.080 N GNG7 n/a
5 TRCN0000008812 GAGCGCATCAAGGTCTCCAAA pLKO.1 416 CDS 100% 4.950 3.465 N GNG7 n/a
6 TRCN0000036814 CTCATGAGCTACTGTGAGCAA pLKO.1 449 CDS 100% 2.640 1.848 N GNG7 n/a
7 TRCN0000036816 TGAGCGCATCAAGGTCTCCAA pLKO.1 415 CDS 100% 2.640 1.848 N GNG7 n/a
8 TRCN0000008810 AGGACAAGAAACCTTGTATTA pLKO.1 525 CDS 100% 1.320 0.924 N GNG7 n/a
9 TRCN0000036818 GCTGGTGGAACAGCTACGCAT pLKO.1 382 CDS 100% 0.880 0.616 N GNG7 n/a
10 TRCN0000008813 CCCTTTAAGGACAAGAAACCT pLKO.1 518 CDS 100% 3.000 1.800 N GNG7 n/a
11 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 1616 3UTR 100% 5.625 2.813 Y KLHL30 n/a
12 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 1616 3UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017026606.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000488795 ACAACACCTGGCAACCAGGGGCAC pLX_317 100% 100% 100% V5 (not translated due to prior stop codon) n/a
2 TRCN0000489789 AGTCACTTGGGAACTCGTTTGACT pLX_317 100% 99.5% 98.5% V5 204_205insG n/a
3 ccsbBroadEn_13862 pDONR223 100% 99.5% 87.1% None 183delC n/a
4 ccsbBroad304_13862 pLX_304 0% 99.5% 87.1% V5 (not translated due to frame shift) 183delC n/a
5 TRCN0000468280 CCGAGACCGTTCAATCCATCCATT pLX_317 100% 99.5% 87.1% V5 (not translated due to frame shift) 183delC n/a
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