Construct: ORF TRCN0000468289
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008973.1_s317c1
- Derived from:
- ccsbBroadEn_02682
- DNA Barcode:
- AACCAAGTGTTCCATATACCACAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GABARAPL2 (11345)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468289
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11345 | GABARAPL2 | GABA type A receptor associ... | NM_007285.7 | 100% | 100% | |
| 2 | mouse | 93739 | Gabarapl2 | gamma-aminobutyric acid (GA... | NM_026693.5 | 92.8% | 100% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 417
- ORF length:
- 351
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaAAAAG 61 TTAGCATGAA GTGGATGTTC AAGGAGGACC ACTCGCTGGA ACACAGATGC GTGGAGTCCG 121 CGAAGATTCG AGCGAAATAT CCCGACAGGG TTCCGGTGAT TGTGGAAAAG GTCTCAGGCT 181 CTCAGATTGT TGACATTGAC AAACGGAAGT ACTTGGTTCC ATCTGATATC ACTGTGGCTC 241 AGTTCATGTG GATCATCAGG AAAAGGATCC AGCTTCCTTC TGAAAAGGCG ATCTTCCTGT 301 TTGTGGATAA GACAGTCCCA CAGTCCAGCC TAACTATGGG ACAGCTTTAC GAGAAGGAAA 361 AAGATGAAGA TGGATTCTTA TATGTGGCCT ACAGCGGAGA GAACACTTTT GGCTTCTGCC 421 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 481 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 541 ATATATCTTG TGGAAAGGAC GAAACCAAGT GTTCCATATA CCACACACGC GTTAAGTCga 601 caatcaacct ctggattaca aaatttgtga aagatt