Construct: ORF TRCN0000468299
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005523.1_s317c1
- Derived from:
- ccsbBroadEn_11790
- DNA Barcode:
- GCCTTAGACCTTAACTAATAAATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SEC31B (25956)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468299
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 25956 | SEC31B | SEC31 homolog B, COPII coat... | NM_015490.4 | 29.3% | 29.1% | (many diffs) |
| 2 | mouse | 240667 | Sec31b | Sec31 homolog B (S. cerevis... | NM_001033343.1 | 26% | 26.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1110
- ORF length:
- 1044
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gctgaaggaa cttgagcggc cagctgtcca ggcatggagc ccagccagcc 121 aatacccttt gtatctggcc acaggaacat ctgcccaaca gctagattcc tccttcagca 181 caaatggcac attggaaata tttgaggttg atttcaggga cccttctctg gacttgaaac 241 acagaggagt cctttctgcc ttgagcaggt ttcacaagct ggtctggggg agctttggca 301 gtgggcttct ggaaagctcc ggggttattg ctggcggcgg ggacaatggc atgcttattc 361 tatacaatgt gacccacatc ctgtcttcgg ggaaggagcc tgtgattgct cagaaacaga 421 agcacacggg ggctgtcaga gccctcgact ttaatccttt ccagggcaac ctcctggctt 481 caggggccag cgattctgaa atcttcattt gggatctgaa taacttgaat gtgccaatga 541 ccctgggatc caagtcacag cctccagagg acatcaaggc actgtcttgg aaccggcaag 601 cccaacacat tctgtcttct gctcacccca gtggcaaggc agttgtgtgg gatctcagga 661 agaatgaacc tatcatcaaa gtcagtgatc acagcaacag gatgcactgc tcaggcctgg 721 cctggcatcc tgacatagcc acccagttag tgctgtgctc agaggatgat cgacttcccg 781 tgattcagct gtgggacttg cgctttgccT CCTCGCCCTT GAAGGTGCTG GAGAGCCACA 841 GCAGGGGGAT CTTGTCAGTG TCATGGAGCC AGGCTGATGC TGAGCTGCTG CTCACTAGTG 901 CTAAGGACAG CCAGATCTTG TGCCGGAACC TGGGGAGCAG TGAGGTGGTA TATAAGCTAC 961 CAACACAGAG CAGCTGGTGC TTTGATGTGC AGTGGTGCCC TCGGGACCCT TCAGTGTTCT 1021 CTGCTGCCTC CTTCAACGGC TGGATCAGTT TGTACGCTGT GATGGGTAGG AGCTGGGAAG 1081 TCCAGCACAT GAGACAGGCT GACAAGGTTT GCCCAACTTT CTTGTACAAA GTGGTTGATA 1141 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1201 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGCCTT 1261 AGACCTTAAC TAATAAATTA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1321 tgaaagatt