Construct: ORF TRCN0000468310
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005604.1_s317c1
- Derived from:
- ccsbBroadEn_06288
- DNA Barcode:
- TTGCGCCTTAATCATCCCAAACTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GMDS (2762)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468310
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2762 | GMDS | GDP-mannose 4,6-dehydratase | NM_001500.4 | 99.9% | 100% | 798T>C |
| 2 | human | 2762 | GMDS | GDP-mannose 4,6-dehydratase | XM_011514500.1 | 91.5% | 90.8% | (many diffs) |
| 3 | human | 2762 | GMDS | GDP-mannose 4,6-dehydratase | NM_001253846.1 | 91.4% | 90.8% | (many diffs) |
| 4 | human | 2762 | GMDS | GDP-mannose 4,6-dehydratase | XM_011514502.3 | 89.2% | 88.7% | (many diffs) |
| 5 | human | 2762 | GMDS | GDP-mannose 4,6-dehydratase | XM_017010752.1 | 73.9% | 67.7% | (many diffs) |
| 6 | human | 2762 | GMDS | GDP-mannose 4,6-dehydratase | XM_006715066.3 | 72.9% | 68.4% | (many diffs) |
| 7 | human | 2762 | GMDS | GDP-mannose 4,6-dehydratase | XM_011514503.3 | 68.7% | 56.2% | (many diffs) |
| 8 | human | 2762 | GMDS | GDP-mannose 4,6-dehydratase | XM_011514505.2 | 64.3% | 56.2% | (many diffs) |
| 9 | human | 2762 | GMDS | GDP-mannose 4,6-dehydratase | XM_011514506.2 | 63.6% | 56% | (many diffs) |
| 10 | human | 2762 | GMDS | GDP-mannose 4,6-dehydratase | XR_001743349.2 | 61.9% | (many diffs) | |
| 11 | human | 2762 | GMDS | GDP-mannose 4,6-dehydratase | XM_011514507.2 | 59.6% | 58.6% | 640_641ins128;666_667ins322 |
| 12 | human | 2762 | GMDS | GDP-mannose 4,6-dehydratase | XR_001743350.2 | 12.4% | (many diffs) | |
| 13 | mouse | 218138 | Gmds | GDP-mannose 4, 6-dehydratase | NM_146041.2 | 89.3% | 97% | (many diffs) |
| 14 | mouse | 218138 | Gmds | GDP-mannose 4, 6-dehydratase | XM_006516656.2 | 70.1% | 75.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1182
- ORF length:
- 1116
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc acacgcaccg gcacgctgcc ccagcgcccg gggctccggg gacggcgaga 121 tgggcaagcc caggaacgtg gcgctcatca ccggtatcac aggccaggat ggttcctacc 181 tggctgagtt cctgctggag aaaggctatg aggtccatgg aattgtacgg cggtccagtt 241 catttaatac gggtcgaatt gagcatctgt ataagaatcc ccaggctcac attgaaggaa 301 acatgaagtt gcactatggc gatctcactg acagtacctg ccttgtgaag atcattaatg 361 aagtaaagcc cacagagatc tacaaccttg gagcccagag ccacgtcaaa atttcctttg 421 acctcgctga gtacactgcg gacgttgacg gagttggcac tctacgactt ctagatgcag 481 ttaagacttg tggccttatc aactctgtga agttctacca agcctcaaca agtgaacttt 541 atgggaaagt gcaggaaata ccccagaagg agaccacccc tttctatccc cggtcaccct 601 atggggcagc aaaactctat gcctattgga ttgtggtgaa cttccgtgag gcgtataatc 661 tctttgcagt gaacggcatt ctcttcaatc atgagagtcc cagaagagga gctaatttcg 721 ttactcgaaa aattagccgg tcagtagcta agatttacct tGGACAACTG GAATGTTTCA 781 GTTTGGGAAA TCTGGATGCC AAACGAGATT GGGGCCATGC CAAGGACTAT GTGGAGGCTA 841 TGTGGTTGAT GTTGCAGAAT GACGAGCCGG AGGACTTCGT TATAGCTACT GGGGAGGTCC 901 ATAGTGTCCG GGAATTTGTC GAGAAATCAT TCTTGCACAT TGGAAAAACC ATTGTGTGGG 961 AAGGAAAGAA TGAAAATGAA GTGGGCAGAT GTAAAGAGAC CGGCAAAGTT CACGTGACTG 1021 TGGATCTCAA GTACTACCGG CCAACTGAAG TGGACTTTCT GCAGGGCGAC TGCACCAAAG 1081 CGAAACAGAA GCTGAACTGG AAGCCCCGGG TCGCTTTCGA TGAGCTGGTG AGGGAGATGG 1141 TGCACGCCGA CGTGGAGCTC ATGAGGACAA ACCCCAATGC CTGCCCAACT TTCTTGTACA 1201 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1261 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1321 AGGACGATTG CGCCTTAATC ATCCCAAACT AACGCGTTAA GTCgacaatc aacctctgga 1381 ttacaaaatt tgtgaaagat t