Construct: ORF TRCN0000468311
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017556.1_s317c1
- Derived from:
- ccsbBroadEn_06619
- DNA Barcode:
- ACCCCAGCCTCCGTAGTACGATAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NCF2 (4688)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468311
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4688 | NCF2 | neutrophil cytosolic factor 2 | NM_000433.3 | 99.8% | 99.6% | 542A>G;606G>A;1167C>A |
| 2 | human | 4688 | NCF2 | neutrophil cytosolic factor 2 | NM_001127651.3 | 99.8% | 99.6% | 542A>G;606G>A;1167C>A |
| 3 | human | 4688 | NCF2 | neutrophil cytosolic factor 2 | XM_011509580.1 | 99.8% | 99.6% | 542A>G;606G>A;1167C>A |
| 4 | human | 4688 | NCF2 | neutrophil cytosolic factor 2 | XM_011509581.1 | 99.8% | 99.6% | 542A>G;606G>A;1167C>A |
| 5 | human | 4688 | NCF2 | neutrophil cytosolic factor 2 | XM_005245207.1 | 93% | 92.9% | 500_501ins108;1059C>A |
| 6 | human | 4688 | NCF2 | neutrophil cytosolic factor 2 | NM_001190794.1 | 91.2% | 91% | (many diffs) |
| 7 | human | 4688 | NCF2 | neutrophil cytosolic factor 2 | NM_001190789.1 | 84.5% | 84.4% | 365_366ins243;924C>A |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1644
- ORF length:
- 1578
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc cctggtggag gccatcagcc tctggaatga aggggtgctg gcagcggaca 121 agaaggactg gaagggagcc ctggatgcct tcagtgccgt ccaggacccc cactcccgga 181 tttgcttcaa cattggctgc atgtacacta tcctgaagaa catgactgaa gcagagaagg 241 cctttaccag aagcattaac cgagacaagc acttggcagt ggcttacttc caacgaggga 301 tgctctacta ccagacagag aaatatgatt tggctatcaa agaccttaaa gaagccttga 361 ttcagcttcg agggaaccag ctgatagact ataagatcct ggggctccag ttcaagctgt 421 ttgcctgtga ggtgttatat aacattgctt tcatgtatgc caagaaggag gaatggaaaa 481 aagctgaaga acagttagca ttggccacga gcatgaagtc tgagcccaga cattccaaaa 541 tcgacaaggc gatggagtgt gtctggaagc agaagctata tgagccagtg gtgatccctg 601 tgggcaggct gtttcgacca aatgagagac aagtggctca gctggccaag aaggattacc 661 taggcaaggc aacggtcgtg gcatctgtgg tggatcaaga cagtttctct gggtttgccc 721 ctctgcaacc acaggcagct gagcctccac ccagaccgaa aaccccagag atcttcaggg 781 ctctggaagg ggaggctcac cgtgtgctat ttgggtttgt gcctgagaca aaagaagagc 841 tccaggtcat gccagggaac attgtctttg tcttgaagaa gggcaatgat aactgggcca 901 cggtcatgtt caacgggcag aaggggcttg ttccctgcaa ctaccttgaa ccagttgagc 961 tgcggatcca ccctcagcag cagccccagg aggaaagctc tccgcagtcc gacatcccag 1021 ctcctcctag ttccaaagcc cctggaagac cccagctgtc accaggccag aaacaaaaag 1081 aagagcctaa ggaagtgaag ctcagtgttc ccatgcccta cacactcaag gtgcactaca 1141 agtacacggt agtcatgaag actcagcccg ggctccccta cagccaggtc cgggacatgg 1201 tgtctaagaa actGGAGCTC CGGCTGGAAC AAACTAAGCT GAGCTATCGG CCTCGGGACA 1261 GCAATGAGCT GGTGCCCCTT TCAGAAGACA GCATGAAGGA TGCCTGGGGC CAGGTGAAAA 1321 ACTACTGCCT GACTCTGTGG TGTGAGAACA CAGTGGGTGA CCAAGGCTTT CCAGATGAAC 1381 CCAAGGAAAG TGAAAAAGCT GATGCTAATA ACCAGACAAC AGAACCTCAG CTTAAGAAAG 1441 GCAGCCAAGT GGAGGCACTC TTCAGTTATG AGGCTACCCA ACCAGAGGAC CTGGAGTTTC 1501 AGGAAGGGGA TATAATCCTG GTGTTATCAA AGGTGAATGA AGAATGGCTG GAAGGGGAGT 1561 GCAAAGGGAA GGTGGGCATT TTCCCCAAAG TTTTTGTTGA AGACTGCGCA ACTACAGATT 1621 TGGAAAGCAC TCGGAGAGAA GTCTACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1681 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1741 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA CCCCAGCCTC 1801 CGTAGTACGA TACACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1861 att