Transcript: Human XM_011509580.1

PREDICTED: Homo sapiens neutrophil cytosolic factor 2 (NCF2), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
NCF2 (4688)
Length:
2250
CDS:
114..1694

Additional Resources:

NCBI RefSeq record:
XM_011509580.1
NBCI Gene record:
NCF2 (4688)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011509580.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000038931 CGGGACATGGTGTCTAAGAAA pLKO.1 1239 CDS 100% 5.625 3.938 N NCF2 n/a
2 TRCN0000038932 GCTATCAAAGACCTTAAAGAA pLKO.1 381 CDS 100% 5.625 3.938 N NCF2 n/a
3 TRCN0000038930 GCCAAGAAGGAGGAATGGAAA pLKO.1 507 CDS 100% 4.950 3.465 N NCF2 n/a
4 TRCN0000038933 CCTGGTGTTATCAAAGGTGAA pLKO.1 1565 CDS 100% 4.050 2.835 N NCF2 n/a
5 TRCN0000038929 GCGCAACTACAGATTTGGAAA pLKO.1 1654 CDS 100% 4.950 2.970 N NCF2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011509580.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06619 pDONR223 100% 99.8% 99.6% None 542A>G;606G>A;1167C>A n/a
2 ccsbBroad304_06619 pLX_304 0% 99.8% 99.6% V5 542A>G;606G>A;1167C>A n/a
3 TRCN0000468311 ACCCCAGCCTCCGTAGTACGATAC pLX_317 27.5% 99.8% 99.6% V5 542A>G;606G>A;1167C>A n/a
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