Construct: ORF TRCN0000468318
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008228.1_s317c1
- Derived from:
- ccsbBroadEn_12715
- DNA Barcode:
- GAAAACGACCACTGAGCGTGTTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SYNC (81493)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468318
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 81493 | SYNC | syncoilin, intermediate fil... | XM_024450011.1 | 32.2% | 32% | (many diffs) |
| 2 | human | 81493 | SYNC | syncoilin, intermediate fil... | XM_024450012.1 | 32.2% | 32% | (many diffs) |
| 3 | human | 81493 | SYNC | syncoilin, intermediate fil... | XM_024450013.1 | 32.2% | 32% | (many diffs) |
| 4 | human | 81493 | SYNC | syncoilin, intermediate fil... | XM_024450014.1 | 32.2% | 32% | (many diffs) |
| 5 | human | 81493 | SYNC | syncoilin, intermediate fil... | NM_030786.3 | 31.3% | 31.3% | 1_993del |
| 6 | human | 81493 | SYNC | syncoilin, intermediate fil... | NM_001161708.2 | 27.4% | 25.4% | (many diffs) |
| 7 | human | 81493 | SYNC | syncoilin, intermediate fil... | XM_024450010.1 | 27.1% | 26.9% | (many diffs) |
| 8 | mouse | 68828 | Sync | syncoilin | NM_023485.3 | 27.7% | 28.6% | (many diffs) |
| 9 | mouse | 68828 | Sync | syncoilin | XM_006503373.2 | 27.5% | 28.3% | (many diffs) |
| 10 | mouse | 68828 | Sync | syncoilin | XM_011240608.1 | 16.3% | 15.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 522
- ORF length:
- 453
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcagagagc cgccaggacc tggaggagga gtatgagccT CAGTTCCTGC 121 GGCTCCTAGA GAGGAAAGAA GCTGGGACCA AAGCTCTGCA GAGAACCCAG GCTGAGATCC 181 AGGAAATGAA GGAGGCTCTG AGACCCCTGC AAGCAGAGGC CCGGCAGCTC CGCCTGCAAA 241 ACAGGAACCT GGAGGACCAG ATCGCACTTG TGAGGCAAAA ACGAGATGAA GAGGTGCAGC 301 AGTACAGGGA ACAGCTGGAG GAAATGGAAG AACGCCAGAG GCAGTTAAGA AATGGGGTGC 361 AACTCCAGCA ACAGAAGAAC AAAGAGATGG AACAGCTAAG GCTCAGTCTT GCTGAAGAGC 421 TCTCTACTTA TAAGGCTATG CTACTACCCA AGAGCCTGGA ACAGGCTGAT GCTCCCACTT 481 CTCAGGCAGG TGGAATGGAG ACACAGTCTC AAGGGGCTGT TTTGCCAACT TTCTTGTACA 541 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 601 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 661 AGGACGAGAA AACGACCACT GAGCGTGTTC AACGCGTTAA GTCgacaatc aacctctgga 721 ttacaaaatt tgtgaaagat t