Construct: ORF TRCN0000468326
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016385.1_s317c1
- Derived from:
- ccsbBroadEn_02441
- DNA Barcode:
- TTAGACCTTTGTACCCTGATGACT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TAB1 (10454)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468326
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10454 | TAB1 | TGF-beta activated kinase 1... | NM_006116.3 | 100% | 100% | |
| 2 | human | 10454 | TAB1 | TGF-beta activated kinase 1... | NM_153497.3 | 88.5% | 85.5% | (many diffs) |
| 3 | mouse | 66513 | Tab1 | TGF-beta activated kinase 1... | NM_025609.2 | 88.3% | 97.2% | (many diffs) |
| 4 | mouse | 66513 | Tab1 | TGF-beta activated kinase 1... | XM_006521259.2 | 58.9% | 65% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1578
- ORF length:
- 1512
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcgcagagg aggagcttgc tgcagagtga gcagcagcca agctggacag 121 atgacctgcc tctctgccac ctctctgggg ttggctcagc ctccaaccgc agctactctg 181 ctgatggcaa gggcactgag agccacccgc cagaggacag ctggctcaag ttcaggagtg 241 agaacaactg cttcctgtat ggggtcttca acggctatga tggcaaccga gtgaccaact 301 tcgtggccca gcggctgtcc gcagagctcc tgctgggcca gctgaatgcc gagcacgccg 361 aggccgatgt gcggcgtgtg ctgctgcagg ccttcgatgt ggtggagagg agcttcctgg 421 agtccattga cgacgccttg gctgagaagg caagcctcca gtcgcaattg ccagagggag 481 tccctcagca ccagctgcct cctcagtatc agaagatcct tgagagactc aagacgttag 541 agagggaaat ttcgggaggg gccatggccg ttgtggcggt ccttctcaac aacaagctct 601 acgtcgccaa tgtcggtaca aaccgtgcac ttttatgcaa atcgacagtg gatgggttgc 661 aggtgacaca gctgaacgtg gaccacacca cagagaacga ggatgagctc ttccgtcttt 721 cgcagctggg cttggatgct ggaaagatca agcaggtggg gatcatctgt gggcaggaga 781 gcacccggcg gatcggggat tacaaggtta aatatggcta cacggacatt gaccttctca 841 gcgctgccaa gtccaaacca atcatcgcag agccagaaat ccatggggca cagccgctgg 901 atggggtgac gggcttcttg gtgctgatgt cggaggggtt gtacaaggcc ctagaggcag 961 cccatgggcc tgggcaggcc aaccaggaga ttgctgcgat gattgacact gagtttgcca 1021 agcagacctc cctggacgca gtggcccagg ccgtcgtgga ccgggtgaag cgcatccaca 1081 gcgacacctt cgccagtggt ggggagcgtg ccaggttctg cccccggcac gaggacatga 1141 ccctgctagt gaggaacttt GGCTACCCGC TGGGCGAAAT GAGCCAGCCC ACACCGAGCC 1201 CAGCCCCAGC TGCAGGAGGA CGAGTGTACC CTGTGTCTGT GCCATACTCC AGCGCCCAGA 1261 GCACCAGCAA GACCAGCGTG ACCCTCTCCC TTGTCATGCC CTCCCAGGGC CAGATGGTCA 1321 ACGGGGCTCA CAGTGCTTCC ACCCTGGACG AAGCCACCCC CACCCTCACC AACCAAAGCC 1381 CGACCTTAAC CCTGCAGTCC ACCAACACGC ACACGCAGAG CAGCAGCTCC AGCTCTGACG 1441 GAGGCCTCTT CCGCTCCCGG CCCGCCCACT CGCTCCCGCC TGGCGAGGAC GGTCGTGTTG 1501 AGCCCTATGT GGACTTTGCT GAGTTTTACC GCCTCTGGAG CGTGGACCAT GGCGAGCAGA 1561 GCGTGGTGAC AGCACCGTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1621 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1681 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATTAGACC TTTGTACCCT 1741 GATGACTACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt