Construct: ORF TRCN0000468343

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013124.1_s317c1
Derived from:
ccsbBroadEn_15844
DNA Barcode:
GTTCTTAATCACTCCACCAAGGAC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RTCB (51493)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000468343
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 51493 RTCB RNA 2',3'-cyclic phosphate ... NM_014306.5 99.9% 100% 1044C>T
2 mouse 28088 Rtcb RNA 2',3'-cyclic phosphate ... NM_145422.4 91.7% 99.4% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1581
ORF length:
1515
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgag tcgcagctat aatgatgagc tgcagttctt ggagaagatc aataaaaact
121gctggaggat caagaagggc ttcgtgccca acatgcaggt tgaaggtgtt ttctatgtga
181atgatgctct ggagaaattg atgtttgagg aattaaggaa tgcctgtcga ggtggtggtg
241ttggtggctt cctgccagcc atgaaacaga ttggcaatgt ggcagccctg cctggaattg
301ttcatcgatc tattgggctt cctgatgtcc attcaggata tgggtttgct attgggaaca
361tggcagcctt tgatatgaat gaccctgaag cagtagtatc cccaggtggt gtcgggtttg
421acatcaactg tggtgtccgc ttgctaagaa ccaatttaga tgaaagtgat gtccagcctg
481tgaaggagca acttgcccaa gctatgtttg accacattcc tgttggggtg gggtcaaaag
541gtgtcatccc aatgaatgcc aaagacttgg aggaggcctt ggagatgggg gtggactggt
601ccttaagaga agggtatgcc tgggctgaag acaaggagca ctgcgaggag tacggaagga
661tgctgcaggc tgaccccaat aaagtttctg caagggcgaa gaaaagaggc cttcctcagt
721tggggaccct gggagcaggc aaccattatg cagaaatcca ggttgtggat gagattttca
781atgagtatgc tgctaaaaaa atgggcatcg accataaggg acaggtgtgt gtgatgatcc
841acagtggaag cagaggcttg ggccaccaag tagccacaga tgcgctggta gctatggaga
901aggccatgaa gagagacaag attatagtca atgatcggca gttggcttgt gctcgaatcg
961cttccccaga gggtcaagac tatctgaagg gaatggcagc tgctgggaac tatgcctggg
1021tcaaccgctc ttccatgacc ttcttaaccc gtcaggcttt cgccaaggtc ttcaacacaa
1081cccctgatga cttGGACCTA CATGTGATTT ATGATGTTTC TCACAACATT GCCAAAGTGG
1141AGCAGCATGT GGTGGACGGA AAGGAACGGA CACTGTTAGT ACACAGGAAG GGATCCACCC
1201GCGCTTTCCC TCCTCACCAT CCCCTCATTG CTGTTGATTA CCAACTCACT GGACAGCCAG
1261TGCTCATTGG TGGCACCATG GGAACCTGTA GTTATGTTCT TACTGGCACT GAACAGGGCA
1321TGACTGAGAC CTTTGGAACA ACCTGTCATG GAGCGGGCCG TGCATTGTCC CGAGCAAAAT
1381CTCGACGTAA TTTAGATTTC CAGGATGTCT TAGACAAATT GGCAGATATG GGAATTGCGA
1441TCCGTGTTGC CTCACCCAAA CTGGTTATGG AAGAGGCTCC TGAGTCCTAT AAGAATGTGA
1501CAGATGTGGT AAATACCTGC CATGATGCTG GAATCAGCAA GAAAGCCATT AAACTGAGAC
1561CAATTGCTGT GATCAAAGGA TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC
1621CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG
1681AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGTTC TTAATCACTC
1741CACCAAGGAC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)