Construct: ORF TRCN0000468351
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011907.1_s317c1
- Derived from:
- ccsbBroadEn_04287
- DNA Barcode:
- ACCTTCGTCACTCGAACTACTACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HSDL1 (83693)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468351
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 83693 | HSDL1 | hydroxysteroid dehydrogenas... | NM_031463.5 | 100% | 100% | |
| 2 | human | 83693 | HSDL1 | hydroxysteroid dehydrogenas... | XM_005256189.3 | 100% | 100% | |
| 3 | human | 83693 | HSDL1 | hydroxysteroid dehydrogenas... | NM_001146051.1 | 83.3% | 83.3% | 311_312ins165 |
| 4 | mouse | 72552 | Hsdl1 | hydroxysteroid dehydrogenas... | NM_175185.4 | 81.3% | 87.8% | (many diffs) |
| 5 | mouse | 72552 | Hsdl1 | hydroxysteroid dehydrogenas... | XM_006531396.1 | 81.3% | 87.8% | (many diffs) |
| 6 | mouse | 72552 | Hsdl1 | hydroxysteroid dehydrogenas... | XM_006531397.3 | 81.3% | 87.8% | (many diffs) |
| 7 | mouse | 72552 | Hsdl1 | hydroxysteroid dehydrogenas... | XM_006531395.3 | 78.9% | 84.6% | (many diffs) |
| 8 | mouse | 72552 | Hsdl1 | hydroxysteroid dehydrogenas... | XM_006531394.3 | 76.9% | 83% | (many diffs) |
| 9 | mouse | 72552 | Hsdl1 | hydroxysteroid dehydrogenas... | XM_006531398.1 | 75.1% | 81.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1059
- ORF length:
- 990
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggctgctgtt gacagtttct acctcttgta cagggaaatc gccaggtctt 121 gcaattgcta tatggaagct ctagctttgg ttggagcctg gtatacggcc agaaaaagca 181 tcactgtcat ctgtgacttt tacagcctga tcaggctgca ttttatcccc cgcctgggga 241 gcagagcaga cttgatcaag cagtatggaa gatgggccgt tgtcagcggt gcaacagatg 301 ggattggaaa agcctacgct gaagagttag caagccgagg tctcaatata atcctgatta 361 gtcggaacga ggagaagttg caggttgttg ctaaagacat agccgacacg tacaaagtgg 421 aaactgatat tatagttgcg gacttcagca gcggtcgtga gatctacctt ccaattcgag 481 aagccctgaa ggacaaagac gttggcatct tggtaaataa cgtgggtgtg ttttatccct 541 acccgcagta tttcactcag ctgtccgagg acaagctctg ggacatcata aatgtgaaca 601 ttgccgccgc tagtttgatg gtccatgttg tgttaccggg aatggtggag agaaagaaag 661 gtgccatcgt cacgatctct tctggctcct gctgcaaacc cactccTCAG CTGGCTGCAT 721 TTTCTGCTTC TAAGGCTTAT TTAGACCACT TCAGCAGAGC CTTGCAATAT GAATATGCCT 781 CTAAAGGAAT CTTTGTACAG AGTCTAATCC CTTTCTATGT AGCCACCAGC ATGACAGCAC 841 CCAGCAACTT TCTGCACAGG TGCTCGTGGT TGGTGCCTTC GCCAAAAGTC TATGCACATC 901 ATGCTGTTTC TACTCTTGGG ATTTCCAAAA GGACCACAGG ATATTGGTCC CATTCTATTC 961 AGTTTCTTTT TGCACAGTAT ATGCCTGAAT GGCTCTGGGT GTGGGGAGCA AATATTCTCA 1021 ACCGTTCACT ACGTAAGGAA GCCTTATCCT GCACAGCCTT GCCAACTTTC TTGTACAAAG 1081 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1141 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1201 ACGAACCTTC GTCACTCGAA CTACTACGAC GCGTTAAGTC gacaatcaac ctctggatta 1261 caaaatttgt gaaagatt