Transcript: Human NM_031463.5

Homo sapiens hydroxysteroid dehydrogenase like 1 (HSDL1), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-05-02
Taxon:
Homo sapiens (human)
Gene:
HSDL1 (83693)
Length:
3648
CDS:
167..1159

Additional Resources:

NCBI RefSeq record:
NM_031463.5
NBCI Gene record:
HSDL1 (83693)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_031463.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000028472 CGTTGGCATCTTGGTAAATAA pLKO.1 598 CDS 100% 15.000 21.000 N HSDL1 n/a
2 TRCN0000436560 TGGATGGCACAGGATCAATAA pLKO_005 1341 3UTR 100% 13.200 18.480 N HSDL1 n/a
3 TRCN0000028394 CCTACGCTGAAGAGTTAGCAA pLKO.1 411 CDS 100% 3.000 4.200 N HSDL1 n/a
4 TRCN0000433543 ACTAAGATTGCTGGTACTATT pLKO_005 1598 3UTR 100% 13.200 10.560 N HSDL1 n/a
5 TRCN0000420742 ATGATTTGTCAGGTCTTATAA pLKO_005 1474 3UTR 100% 15.000 10.500 N HSDL1 n/a
6 TRCN0000028433 GCAATATGAATATGCCTCTAA pLKO.1 862 CDS 100% 4.950 3.465 N HSDL1 n/a
7 TRCN0000028451 GCTAGTTTGATGGTCCATGTT pLKO.1 707 CDS 100% 4.950 3.465 N HSDL1 n/a
8 TRCN0000028462 GCTGTTTCTACTCTTGGGATT pLKO.1 1001 CDS 100% 4.050 2.835 N HSDL1 n/a
9 TRCN0000021429 CACACCTGTAATCCCAGCATT pLKO.1 3155 3UTR 100% 4.950 2.475 Y ERN2 n/a
10 TRCN0000138998 CACACCTGTAATCCCAGCATT pLKO.1 3155 3UTR 100% 4.950 2.475 Y P3H4 n/a
11 TRCN0000344020 CACACCTGTAATCCCAGCATT pLKO_005 3155 3UTR 100% 4.950 2.475 Y P3H4 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_031463.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04287 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_04287 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000468351 ACCTTCGTCACTCGAACTACTACG pLX_317 35.8% 100% 100% V5 n/a
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