Construct: ORF TRCN0000468362
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007721.1_s317c1
- Derived from:
- ccsbBroadEn_09407
- DNA Barcode:
- GCATGTTCTGACATTGTGCCTACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MRFAP1L1 (114932)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468362
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 114932 | MRFAP1L1 | Morf4 family associated pro... | NM_203462.3 | 99.4% | 99.2% | 97A>C;114C>T |
| 2 | human | 114932 | MRFAP1L1 | Morf4 family associated pro... | XM_005247932.2 | 99.4% | 99.2% | 97A>C;114C>T |
| 3 | human | 93621 | MRFAP1 | Morf4 family associated pro... | NM_001272053.1 | 87.5% | 84.2% | (many diffs) |
| 4 | human | 93621 | MRFAP1 | Morf4 family associated pro... | NM_033296.2 | 87.5% | 84.2% | (many diffs) |
| 5 | human | 93621 | MRFAP1 | Morf4 family associated pro... | NM_001272054.1 | 72.9% | 64.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 447
- ORF length:
- 381
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcg gcccctggac atagacgagg tggaagcgcc TGAGGAAGTG GAGGTGCTGG 121 AGCCCGAGGA GGATTTCGAG CAGTTCCTGC TCCCGGTCAT CCACGAGATG CGCGAGGATA 181 TCGCGTCTCT TATACGCGAG CACGGGCGGG CGTACCTGCG GACCAGGAGC AAGCTGTGGG 241 AGATGGACAA TATGCTTATC CAGATCAAAA CGCAGGTGGA GGCCTCGGAG GAGAGCGCCC 301 TCAATCACGT GCAGCACCCG AGTGGCGAAG CCGACGAGAG AGTGTCGGAG TTGTGCGAGA 361 AGGCTGAGGA GAAAGCCAAG GAGATTGCGA AGATGGCAGA GATGCTGGTC GAGCTCGTCT 421 GGCGAATAGA GAGAAGCGAG TCTTCTTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 481 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 541 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGCATGTTC 601 TGACATTGTG CCTACGACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 661 aagatt