Transcript: Human NM_203462.3

Homo sapiens Morf4 family associated protein 1 like 1 (MRFAP1L1), mRNA.

Source:
NCBI, updated 2019-05-02
Taxon:
Homo sapiens (human)
Gene:
MRFAP1L1 (114932)
Length:
1578
CDS:
237..620

Additional Resources:

NCBI RefSeq record:
NM_203462.3
NBCI Gene record:
MRFAP1L1 (114932)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_203462.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000161514 GCCTGGTGAAGAATGTATTAA pLKO.1 1052 3UTR 100% 15.000 10.500 N MRFAP1L1 n/a
2 TRCN0000165585 GAGGACATCGCGTCTCTTATA pLKO.1 345 CDS 100% 13.200 9.240 N MRFAP1L1 n/a
3 TRCN0000161122 GCAGTTTGAATAGGGACTATT pLKO.1 1270 3UTR 100% 13.200 9.240 N MRFAP1L1 n/a
4 TRCN0000163216 GAATAGAGAGAAGCGAGTCTT pLKO.1 595 CDS 100% 4.950 3.465 N MRFAP1L1 n/a
5 TRCN0000165794 CGAATAGAGAGAAGCGAGTCT pLKO.1 594 CDS 100% 2.640 1.848 N MRFAP1L1 n/a
6 TRCN0000159501 CAATATGCTTATCCAGATCAA pLKO.1 419 CDS 100% 4.950 2.970 N MRFAP1L1 n/a
7 TRCN0000165674 GAGGAGAAAGCCAAGGAGATT pLKO.1 537 CDS 100% 4.950 2.970 N MRFAP1L1 n/a
8 TRCN0000161294 GATGGACAATATGCTTATCCA pLKO.1 413 CDS 100% 3.000 1.800 N MRFAP1L1 n/a
9 TRCN0000166100 GAAAGCCAAGGAGATTGCGAA pLKO.1 542 CDS 100% 2.640 1.584 N MRFAP1L1 n/a
10 TRCN0000141423 CTGTGGGAGATGGACAATATG pLKO.1 405 CDS 100% 13.200 6.600 Y MRFAP1 n/a
11 TRCN0000122857 GCTGTGGGAGATGGACAATAT pLKO.1 404 CDS 100% 13.200 6.600 Y MRFAP1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_203462.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04670 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_04670 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000465464 GACATATTAACCCTTGTCTACTCG pLX_317 66.8% 100% 100% V5 n/a
4 ccsbBroadEn_09407 pDONR223 100% 99.4% 99.2% None 97A>C;114C>T n/a
5 ccsbBroad304_09407 pLX_304 0% 99.4% 99.2% V5 97A>C;114C>T n/a
6 TRCN0000468362 GCATGTTCTGACATTGTGCCTACG pLX_317 89% 99.4% 99.2% V5 97A>C;114C>T n/a
7 ccsbBroadEn_04605 pDONR223 100% 88% 85% None (many diffs) n/a
8 ccsbBroad304_04605 pLX_304 0% 88% 85% V5 (many diffs) n/a
9 TRCN0000474405 TTACTCACTTTATGACCACAACAA pLX_317 100% 88% 85% V5 (many diffs) n/a
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