Transcript: Human NM_033296.2

Homo sapiens Morf4 family associated protein 1 (MRFAP1), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-05-04
Taxon:
Homo sapiens (human)
Gene:
MRFAP1 (93621)
Length:
2184
CDS:
773..1156

Additional Resources:

NCBI RefSeq record:
NM_033296.2
NBCI Gene record:
MRFAP1 (93621)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_033296.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000143197 GAGCTGGAGAATGGATAGTAT pLKO.1 1692 3UTR 100% 5.625 4.500 N MRFAP1 n/a
2 TRCN0000141085 CTGTCTGCATTGGACCCTAAA pLKO.1 1995 3UTR 100% 10.800 7.560 N MRFAP1 n/a
3 TRCN0000142686 CCAAAGGAGGAGAGATGTGTT pLKO.1 1748 3UTR 100% 4.950 3.465 N MRFAP1 n/a
4 TRCN0000145358 GACCTCAATATGAGTTTCGAT pLKO.1 1317 3UTR 100% 3.000 2.100 N MRFAP1 n/a
5 TRCN0000144428 CAATATGCTCATCCAGATCAA pLKO.1 955 CDS 100% 4.950 2.970 N MRFAP1 n/a
6 TRCN0000143295 GCTCTGTCTCACTTAACACTA pLKO.1 1269 3UTR 100% 4.950 2.970 N MRFAP1 n/a
7 TRCN0000143790 GACAATATGCTCATCCAGATC pLKO.1 953 CDS 100% 4.050 2.430 N MRFAP1 n/a
8 TRCN0000143736 GAGATGGACAATATGCTCATC pLKO.1 947 CDS 100% 4.050 2.430 N MRFAP1 n/a
9 TRCN0000141423 CTGTGGGAGATGGACAATATG pLKO.1 941 CDS 100% 13.200 6.600 Y MRFAP1 n/a
10 TRCN0000122857 GCTGTGGGAGATGGACAATAT pLKO.1 940 CDS 100% 13.200 6.600 Y MRFAP1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_033296.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04605 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_04605 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000474405 TTACTCACTTTATGACCACAACAA pLX_317 100% 100% 100% V5 n/a
4 ccsbBroadEn_04670 pDONR223 100% 88% 85% None (many diffs) n/a
5 ccsbBroad304_04670 pLX_304 0% 88% 85% V5 (many diffs) n/a
6 TRCN0000465464 GACATATTAACCCTTGTCTACTCG pLX_317 66.8% 88% 85% V5 (many diffs) n/a
7 ccsbBroadEn_09407 pDONR223 100% 87.5% 84.2% None (many diffs) n/a
8 ccsbBroad304_09407 pLX_304 0% 87.5% 84.2% V5 (many diffs) n/a
9 TRCN0000468362 GCATGTTCTGACATTGTGCCTACG pLX_317 89% 87.5% 84.2% V5 (many diffs) n/a
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