Construct: ORF TRCN0000468365
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011426.1_s317c1
- Derived from:
- ccsbBroadEn_09357
- DNA Barcode:
- CCGTCTTACGCATTTGTGTCGCAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MYL10 (93408)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468365
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 93408 | MYL10 | myosin light chain 10 | NM_138403.5 | 99.8% | 100% | 603A>G |
| 2 | human | 93408 | MYL10 | myosin light chain 10 | XM_017012793.1 | 64.8% | 65% | 0_1ins135;36_37ins102;366A>G |
| 3 | mouse | 59310 | Myl10 | myosin, light chain 10, reg... | XM_017321052.1 | 59.7% | 60.1% | (many diffs) |
| 4 | mouse | 59310 | Myl10 | myosin, light chain 10, reg... | XM_017321050.1 | 59% | 51.9% | (many diffs) |
| 5 | mouse | 59310 | Myl10 | myosin, light chain 10, reg... | NM_021611.3 | 57.7% | 64.6% | (many diffs) |
| 6 | mouse | 59310 | Myl10 | myosin, light chain 10, reg... | NM_001085387.2 | 51.2% | 57.5% | (many diffs) |
| 7 | mouse | 59310 | Myl10 | myosin, light chain 10, reg... | NM_001303021.1 | 51.2% | 57.5% | (many diffs) |
| 8 | mouse | 59310 | Myl10 | myosin, light chain 10, reg... | NM_001303019.1 | 36.2% | 31.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 744
- ORF length:
- 678
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt gcttaggctg gtctcaaact cctggcctca agtgatcctc cctcctcggc 121 ctcccaaagt gctgggatta caggcaccga gaagagctcg gaaaagagca gaaggcaccg 181 ccagctccaa cgtcttctcc atgtttgatc agtcccagat ccaggagttt aaagagagtc 241 tggctctgtc gcccaggctg gagcgcaatg gcatgatctc ggctcactgc aacctctgcc 301 tcacgggttc aagcaattct cctgcctcag cctccCAAGC TTTCACCATC ATGGACCAGA 361 ACCGTGATGG CTTCATCGAC AAAGAGGACT TGAGGGACAC CTTTGCCGCG CTGGGCCGCA 421 TCAATGTCAA GAACGAGGAA CTGGAGGCCA TGGTGAAGGA GGCCCCCGGA CCCATCAACT 481 TCACGGTGTT CCTGACCATG TTTGGGGAGA AGCTGAAGGG CACGGACCCA GAGGAGACCA 541 TTCTCCACGC CTTCAAAGTG TTCGACACTG AAGGGAAAGG TTTCGTCAAG GCCGATGTCA 601 TCAAAGAAAA ACTTATGACC CAGGCAGACC GCTTCAGTGA GGAGGAGGTC AAGCAGATGT 661 TTGCAGCGTT TCCCCCAGAT GTGTGCGGCA ACCTGGACTA CAGAAACCTG TGCTACGTCA 721 TCACTCACGG TGAAGAGAAG GATTACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 781 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 841 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC CGTCTTACGC 901 ATTTGTGTCG CACACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 961 att