Transcript: Mouse XM_017321050.1

PREDICTED: Mus musculus myosin, light chain 10, regulatory (Myl10), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Myl10 (59310)
Length:
1880
CDS:
355..1053

Additional Resources:

NCBI RefSeq record:
XM_017321050.1
NBCI Gene record:
Myl10 (59310)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_017321050.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000434948 GCTGCACTGGGTCGCATAAAT pLKO_005 604 CDS 100% 15.000 10.500 N Myl10 n/a
2 TRCN0000104751 CTGCACGCCTTCAAAGTGTTT pLKO.1 742 CDS 100% 4.950 3.465 N Myl10 n/a
3 TRCN0000104752 GCCAGATGTCTGTGGCAACTT pLKO.1 904 CDS 100% 4.950 3.465 N Myl10 n/a
4 TRCN0000413829 TAAATGTCAAGAACGAGGAAT pLKO_005 620 CDS 100% 4.950 3.465 N Myl10 n/a
5 TRCN0000104750 CAGGAATCTGTGCTACGTCAT pLKO.1 931 CDS 100% 4.050 2.835 N Myl10 n/a
6 TRCN0000421556 CCGCGATGGCTTCATTGACAA pLKO_005 561 CDS 100% 4.950 2.970 N Myl10 n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017321050.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_09357 pDONR223 100% 59% 51.9% None (many diffs) n/a
2 ccsbBroad304_09357 pLX_304 0% 59% 51.9% V5 (many diffs) n/a
3 TRCN0000468365 CCGTCTTACGCATTTGTGTCGCAC pLX_317 60% 59% 51.9% V5 (many diffs) n/a
Download CSV