Construct: ORF TRCN0000468368

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016391.3_s317c1
Derived from:
ccsbBroadEn_01280
DNA Barcode:
GGGGTGGGGATGTTACTTTGTGGC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PRKAR1A (5573)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000468368
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5573 PRKAR1A protein kinase cAMP-depende... NM_001276289.1 100% 100%
2 human 5573 PRKAR1A protein kinase cAMP-depende... NM_001278433.1 100% 100%
3 human 5573 PRKAR1A protein kinase cAMP-depende... NM_001369389.1 100% 100%
4 human 5573 PRKAR1A protein kinase cAMP-depende... NM_001369390.1 100% 100%
5 human 5573 PRKAR1A protein kinase cAMP-depende... NM_002734.4 100% 100%
6 human 5573 PRKAR1A protein kinase cAMP-depende... NM_212471.2 100% 100%
7 human 5573 PRKAR1A protein kinase cAMP-depende... NM_212472.2 100% 100%
8 human 5573 PRKAR1A protein kinase cAMP-depende... XM_011524984.3 100% 100%
9 human 5573 PRKAR1A protein kinase cAMP-depende... NM_001276290.1 87.3% 85.4% (many diffs)
10 mouse 19084 Prkar1a protein kinase, cAMP depend... NM_001313973.1 88.1% 96.8% (many diffs)
11 mouse 19084 Prkar1a protein kinase, cAMP depend... NM_001313974.1 88.1% 96.8% (many diffs)
12 mouse 19084 Prkar1a protein kinase, cAMP depend... NM_001313975.1 88.1% 96.8% (many diffs)
13 mouse 19084 Prkar1a protein kinase, cAMP depend... NM_001313976.1 88.1% 96.8% (many diffs)
14 mouse 19084 Prkar1a protein kinase, cAMP depend... NM_021880.3 88.1% 96.8% (many diffs)
15 mouse 19084 Prkar1a protein kinase, cAMP depend... XM_017314340.1 88.1% 96.8% (many diffs)
16 mouse 19084 Prkar1a protein kinase, cAMP depend... XM_006532509.1 65.8% 72.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1209
ORF length:
1143
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgga gtctggcagt accgccgcca gtgaggaggc acgcagcctt cgagaatgtg
121agctctacgt ccagaagcat aacattcaag cgctgctcaa agattctatt gtgcagttgt
181gcactgctcg acctgagaga cccatggcat tcctcaggga atactttgag aggttggaga
241aggaggaggc aaaacagatt cagaatctgc agaaagcagg cactcgtaca gactcaaggg
301aggatgagat ttctcctcct ccacccaacc cagtggttaa aggtaggagg cgacgaggtg
361ctatcagcgc tgaggtctac acggaggaag atgcggcatc ctatgttaga aaggttatac
421caaaagatta caagacaatg gccgctttag ccaaagccat tgaaaagaat gtgctgtttt
481cacatcttga tgataatgag agaagtgata tttttgatgc catgttttcg gtctccttta
541tcgcaggaga gactgtgatt cagcaaggtg atgaagggga taacttctat gtgattgatc
601aaggagagac ggatgtctat gttaacaatg aatgggcaac cagtgttggg gaaggaggga
661gctttggaga acttgctttg atttatggaa caccgagagc agccactgtc aaagcaaaga
721caaatgtgaa attgtggggc atcgaccgag acagctatag aagaatcctc atgggaagca
781cactgagaaa gcggaagatg tatgaggaat tccTTAGTAA AGTCTCTATT TTAGAGTCTC
841TGGACAAGTG GGAACGTCTT ACGGTAGCTG ATGCATTGGA ACCAGTGCAG TTTGAAGATG
901GGCAGAAGAT TGTGGTGCAG GGAGAACCAG GGGATGAGTT CTTCATTATT TTAGAGGGGT
961CAGCTGCTGT GCTACAACGT CGGTCAGAAA ATGAAGAGTT TGTTGAAGTG GGAAGATTGG
1021GGCCTTCTGA TTATTTTGGT GAAATTGCAC TACTGATGAA TCGTCCTCGT GCTGCCACAG
1081TTGTTGCTCG TGGCCCCTTG AAGTGCGTTA AGCTGGACCG ACCTAGATTT GAACGTGTTC
1141TTGGCCCATG CTCAGACATC CTCAAACGAA ACATCCAGCA GTACAACAGT TTTGTGTCAC
1201TGTCTGTCTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC
1261CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA
1321TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAGGGGTG GGGATGTTAC TTTGTGGCAC
1381GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt

Download FASTA (ORF) (Full)