Construct: ORF TRCN0000468371
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000081.1_s317c1
- Derived from:
- ccsbBroadEn_12136
- DNA Barcode:
- ACCTGGAGGATGCGCACCAAGTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PRPF39 (55015)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468371
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55015 | PRPF39 | pre-mRNA processing factor 39 | NM_017922.4 | 94% | 94% | 1_120del |
| 2 | mouse | 328110 | Prpf39 | pre-mRNA processing factor 39 | NM_177806.3 | 86.7% | 88.4% | (many diffs) |
| 3 | mouse | 328110 | Prpf39 | pre-mRNA processing factor 39 | XM_006516026.3 | 80% | 80.1% | (many diffs) |
| 4 | mouse | 328110 | Prpf39 | pre-mRNA processing factor 39 | XM_006516027.3 | 80% | 80.1% | (many diffs) |
| 5 | mouse | 328110 | Prpf39 | pre-mRNA processing factor 39 | XM_006516028.3 | 80% | 80.1% | (many diffs) |
| 6 | mouse | 328110 | Prpf39 | pre-mRNA processing factor 39 | XM_017315120.1 | 80% | 80.1% | (many diffs) |
| 7 | mouse | 328110 | Prpf39 | pre-mRNA processing factor 39 | XM_006516029.3 | 67.1% | 67.2% | (many diffs) |
| 8 | mouse | 328110 | Prpf39 | pre-mRNA processing factor 39 | XM_006516031.2 | 50.4% | 48.9% | (many diffs) |
| 9 | mouse | 328110 | Prpf39 | pre-mRNA processing factor 39 | XR_001780462.1 | 41.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1956
- ORF length:
- 1887
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaacagtca cctgatgact ctcccaatgt gaatgcatct acagaagaaa 121 ctgaaatggc aagtgctgtg gaccttccag tgacgctgac agaaacagaa gcaaatttcc 181 ctccagaata tgaaaaattt tggaaaactg tagaaaataa tcctcaggat tttacaggct 241 gggtatattt gcttcaatat gtagaacagg agaatcactt gatggctgcc aggaaggcat 301 ttgacagatt tttcatacac tatccgtatt gctatggtta ctggaaaaag tatgcagacc 361 ttgaaaagcg gcacgacaac attaaaccat cagatgaggt ttatcggcgg gggcttcagg 421 caatacctct tagtgttgac ctttggatac attatataaa cttcttaaaa gaaacattgg 481 accctggtga tcctgagaca aacaatacaa taagaggaac ttttgagcat gctgttctag 541 ctgcaggaac agatttccgt tctgacagac tgtgggaaat gtatataaac tgggaaaatg 601 agcagggaaa cctgagagaa gttacagcta tatatgatcg tattcttggt attccaacac 661 agctgtatag tcatcatttt cagagattta aagaacatgt acagaataat ttgcctagag 721 atcttttaac tggtgaacag tttattcagt tgcgaaggga attagcttct gtaaatggtc 781 atagtggtga tgatggtcct cctggtgatg atctaccatc gggaattgaa gacataaccg 841 atcctgcaaa gctaattaca gaaatagaaa acatgagaca tagaatcatt gagattcatc 901 aagaaatgtt taattataat gagcatgaag ttagtaaaag gtggacattt gaagaaggta 961 ttaaaagacc ttactttcat gtgaaacctt tggaaaaggc acaactaaaa aactggaaag 1021 aatacttaga atttgaaatt gaaaatggga ctcatgaacg agttgtggtt ctctttgaaa 1081 gatgtgtcat atcatgtgcc ctctatgagg agttttggat taagtatgcc aagtacatgg 1141 aaaaccatag cattgaagga gtgaggcatg tcttcagcag agcttgtact atacatctcc 1201 caaagaaacc catggtgcat atgctttggg cagcttttga ggaacagcag ggtaatatta 1261 atgaagccag gaatatcttg aaaacatttg aagaatgtgt tctaggattg gcaatggttc 1321 gtttacgaag agtaagttta gaacgacggc atggaaatct ggaagaagct gaacatttgc 1381 ttcaggatgc cattaagaat gccaaatcaa ataatgaatc ttcattttat gctgtcaaac 1441 tagcccggca tcttttcaaa atacagaaaa accttccaaa atcaagaaag gtgcttttgg 1501 aagcaatcga aagagacaaa gagaacacaa agttatacct caatttactt gaaatggaat 1561 atagtggtga cctcaaacaa aatgaagaaa atatcctaaa ttgttttGAC AAAGCTGTAC 1621 ATGGTTCATT ACCTATTAAA ATGAGAATTA CATTTTCTCA GAGAAAAGTG GAATTTCTTG 1681 AAGATTTTGG TTCCGATGTT AATAAGCTTC TGAATGCTTA TGATGAACAT CAAACACTCC 1741 TGAAAGAACA GGATTCTTTA AAAAGGAAAG CAGAAAATGG ATCAGAAGAA CCAGAGGAAA 1801 AGAAAGCACA TACAGAAGAT ACAACTTCAT CATCTACACA GATGATTGAT GGTGATTTAC 1861 AGGCAAACCA AGCTGTATAT AATTATAGTG CGTGGTATCA ATACAATTAT CAGAATCCTT 1921 GGAATTATGG ACAATATTAT CCTCCCCCTC CAACCTTGCC AACTTTCTTG TACAAAGTGG 1981 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 2041 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 2101 AACCTGGAGG ATGCGCACCA AGTCAACGCG TTAAGTCgac aatcaacctc tggattacaa 2161 aatttgtgaa agatt