Construct: ORF TRCN0000468383
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012716.1_s317c1
- Derived from:
- ccsbBroadEn_06646
- DNA Barcode:
- CCCCTCCCAATTCCCCGTTAGACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NNMT (4837)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468383
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4837 | NNMT | nicotinamide N-methyltransf... | NM_001372045.1 | 99.8% | 99.6% | 20C>G |
| 2 | human | 4837 | NNMT | nicotinamide N-methyltransf... | NM_001372046.1 | 99.8% | 99.6% | 20C>G |
| 3 | human | 4837 | NNMT | nicotinamide N-methyltransf... | NM_001372047.1 | 99.8% | 99.6% | 20C>G |
| 4 | human | 4837 | NNMT | nicotinamide N-methyltransf... | NM_006169.3 | 99.8% | 99.6% | 20C>G |
| 5 | human | 4837 | NNMT | nicotinamide N-methyltransf... | NR_164073.1 | 35.1% | (many diffs) | |
| 6 | mouse | 18113 | Nnmt | nicotinamide N-methyltransf... | NM_001311062.1 | 50.7% | 47.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 67
- ORF end:
- 859
- ORF length:
- 792
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaatgg aatcaggctt cacctgcaag gacacctatc taagccattt taaccctcgg 121 gattacctag aaaaatatta caagtttggt tctaggcact ctgcagaaag ccagattctt 181 aagcaccttc tgaaaaatct tttcaagata ttctgcctag acggtgtgaa gggagacctg 241 ctgattgaca tcggctctgg ccccactatc tatcagctcc tctctgcttg tgaatccttt 301 aaggagatcg tcgtcactga ctactcagac cagaacctgc aggagctgga gaagtggctg 361 aagaaagagc cagaggcctt tgactggtcc ccagtggtga cctatgtgtg tgatcttGAA 421 GGGAACAGAG TCAAGGGTCC AGAGAAGGAG GAGAAGTTGA GACAGGCGGT CAAGCAGGTG 481 CTGAAGTGTG ATGTGACTCA GAGCCAGCCA CTGGGGGCCG TCCCCTTACC CCCGGCTGAC 541 TGCGTGCTCA GCACACTGTG TCTGGATGCC GCCTGCCCAG ACCTCCCCAC CTACTGCAGG 601 GCGCTCAGGA ACCTCGGCAG CCTACTGAAG CCAGGGGGCT TCCTGGTGAT CATGGATGCG 661 CTCAAGAGCA GCTACTACAT GATTGGTGAG CAGAAGTTCT CCAGCCTCCC CCTGGGCCGG 721 GAGGCAGTAG AGGCTGCTGT GAAAGAGGCT GGCTACACAA TCGAATGGTT TGAGGTGATC 781 TCGCAAAGTT ATTCTTCCAC CATGGCCAAC AACGAAGGAC TTTTCTCCCT GGTGGCGAGG 841 AAGCTGAGCA GACCCCTGTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT 901 ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA 961 AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACCCCTC CCAATTCCCC 1021 GTTAGACGAC GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt