Construct: ORF TRCN0000468391
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001272.1_s317c1
- Derived from:
- ccsbBroadEn_04119
- DNA Barcode:
- AACACCACTTAACGTATCCTGGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DHRS12 (79758)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468391
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79758 | DHRS12 | dehydrogenase/reductase 12 | NM_001031719.3 | 100% | 100% | |
| 2 | human | 79758 | DHRS12 | dehydrogenase/reductase 12 | NM_024705.2 | 79.2% | 67.8% | (many diffs) |
| 3 | human | 79758 | DHRS12 | dehydrogenase/reductase 12 | NM_001270424.1 | 65.6% | 50.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 879
- ORF length:
- 813
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa tctgcatgta aagactttgt ccctcatgac ttggaggtcc agattcctgg 121 aagagtcttt ttggtcactg gaggaaacag cggcattggc aaagcaactg cccttgaaat 181 cgccaagcga gaacattttt ctgcacattg tggacttgtc tgatcccaag caaatctgga 241 aatttgttga aaatttcaag caggaacata aactccatgt tctgatcaat aatgcaggtt 301 gcatggtcaa taaaagagag ctcacagaag atggacttga aaaaaacttt gctgccaata 361 ctctgggtgt gtacattctc acgaccggcc tgatccctgt gctggagaaa gaacacgacc 421 cccgagtgat aaccgtctcc tcaggaggaa tgttggttca gaaactgaac accaatgatc 481 tccagtccga aagaacacca tttgatggaa ctatggtcta tgcacaaaac aagaggcagc 541 aagtggttct gacggagcgg tgggcccaag ggcacccggc catccatttt tcTTCCATGC 601 ATCCTGGCTG GGCCGACACC CCAGACAGGA ATGAGCAGGA GCTGAGGAAG GTAGTGGGAG 661 AGGCCCAGAC TGCCTCACCA CTCCCCAGGT TTTTGGAAAT AATGATGCAT GAAGGTAAAT 721 GCCAGCCACA AGGACACAGC TCGAATGATC TGGAAGCGTG TTGGAGCAGC GGTGGAGGGG 781 AGCAGAATTC TCTTCCGGAT TGGCCTCACC AACTCCATGA CCTCAGGCAG CTCACCTGGG 841 CTCTCTGCAG CTCTTTCCTC CTCTACAAAC AAGGGAACTG CCCAACTTTC TTGTACAAAG 901 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 961 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1021 ACGAAACACC ACTTAACGTA TCCTGGCAAC GCGTTAAGTC gacaatcaac ctctggatta 1081 caaaatttgt gaaagatt