Transcript: Human NM_024705.2

Homo sapiens dehydrogenase/reductase 12 (DHRS12), transcript variant 2, mRNA.

Source:
NCBI, updated 2018-06-25
Taxon:
Homo sapiens (human)
Gene:
DHRS12 (79758)
Length:
2015
CDS:
118..846

Additional Resources:

NCBI RefSeq record:
NM_024705.2
NBCI Gene record:
DHRS12 (79758)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_024705.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000243368 TGCAGGTTGCATGGTCAATAA pLKO_005 345 CDS 100% 13.200 18.480 N DHRS12 n/a
2 TRCN0000167714 GCACTTTACATATCAATCCAT pLKO.1 1488 3UTR 100% 3.000 4.200 N DHRS12 n/a
3 TRCN0000243365 CAAAGCAACTGCCCTTGAAAT pLKO_005 212 CDS 100% 13.200 10.560 N DHRS12 n/a
4 TRCN0000243367 CCGAAGAGGAGAAACTCATTG pLKO_005 1728 3UTR 100% 10.800 7.560 N DHRS12 n/a
5 TRCN0000243366 CTTGTCTGATCCCAAGCAAAT pLKO_005 267 CDS 100% 10.800 7.560 N DHRS12 n/a
6 TRCN0000243364 TTGATGGAACTATGGTCTATG pLKO_005 554 CDS 100% 10.800 7.560 N DHRS12 n/a
7 TRCN0000173037 CCGCTTCTTTCAAGGTGACTT pLKO.1 801 CDS 100% 4.950 3.465 N DHRS12 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_024705.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04119 pDONR223 100% 79.2% 67.8% None (many diffs) n/a
2 ccsbBroad304_04119 pLX_304 0% 79.2% 67.8% V5 (many diffs) n/a
3 TRCN0000468391 AACACCACTTAACGTATCCTGGCA pLX_317 35.6% 79.2% 67.8% V5 (many diffs) n/a
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