Construct: ORF TRCN0000468400
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010159.1_s317c1
- Derived from:
- ccsbBroadEn_08225
- DNA Barcode:
- CTGCCTGTCACAGATCGGCATATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GOLGA7 (51125)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468400
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51125 | GOLGA7 | golgin A7 | NM_001002296.2 | 99.5% | 99.2% | 7C>G;180C>T |
| 2 | human | 51125 | GOLGA7 | golgin A7 | NM_001362979.1 | 99.5% | 99.2% | 7C>G;180C>T |
| 3 | human | 51125 | GOLGA7 | golgin A7 | NM_001362980.1 | 99.5% | 99.2% | 7C>G;180C>T |
| 4 | human | 51125 | GOLGA7 | golgin A7 | NM_016099.2 | 99.5% | 99.2% | 7C>G;180C>T |
| 5 | human | 51125 | GOLGA7 | golgin A7 | NM_001174124.1 | 94.9% | 88.3% | (many diffs) |
| 6 | human | 51125 | GOLGA7 | golgin A7 | NR_156426.1 | 21.6% | (many diffs) | |
| 7 | human | 51125 | GOLGA7 | golgin A7 | NR_156425.1 | 21.3% | (many diffs) | |
| 8 | human | 51125 | GOLGA7 | golgin A7 | NR_156427.1 | 18.5% | (many diffs) | |
| 9 | mouse | 57437 | Golga7 | golgi autoantigen, golgin s... | NM_001042484.1 | 92.7% | 98.5% | (many diffs) |
| 10 | mouse | 57437 | Golga7 | golgi autoantigen, golgin s... | NM_020585.2 | 92.7% | 98.5% | (many diffs) |
| 11 | mouse | 57437 | Golga7 | golgi autoantigen, golgin s... | XM_006509179.3 | 88.7% | 83.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 477
- ORF length:
- 411
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag ggcgcagcag gcgccggtgt ccggaaaggt gttcattcag cgagactaca 121 gcagtggcac acgctgccag ttccagacca agttccctgc ggagctGGAG AACCGGATTG 181 ATAGGCAGCA GTTTGAAGAA ACAGTTCGAA CTCTAAATAA CCTTTATGCA GAAGCAGAGA 241 AGCTTGGCGG CCAGTCATAT CTCGAAGGTT GTTTGGCTTG TTTAACAGCA TATACCATCT 301 TCCTATGCAT GGAAACTCAT TATGAGAAGG TTCTGAAGAA AGTCTCCAAA TACATTCAAG 361 AGCAGAATGA GAAGATCTAT GCTCCACAAG GCCTCCTCCT GACAGACCCT ATTGAGCGAG 421 GACTGCGAGT TATTGAAATT ACCATTTATG AAGACAGAGG CATGAGCAGT GGAAGATACC 481 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 541 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 601 ATATATCTTG TGGAAAGGAC GACTGCCTGT CACAGATCGG CATATTACGC GTTAAGTCga 661 caatcaacct ctggattaca aaatttgtga aagatt