Construct: ORF TRCN0000468401
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010418.1_s317c1
- Derived from:
- ccsbBroadEn_08028
- DNA Barcode:
- TCCCAACAATTTTAATTGTCCACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AATF (26574)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468401
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26574 | AATF | apoptosis antagonizing tran... | NM_012138.4 | 99.9% | 99.8% | 5C>T |
| 2 | human | 26574 | AATF | apoptosis antagonizing tran... | XM_011524611.2 | 95.6% | 95.5% | 5C>T;1546_1547ins72 |
| 3 | human | 26574 | AATF | apoptosis antagonizing tran... | XR_934439.3 | 75.8% | (many diffs) | |
| 4 | mouse | 56321 | Aatf | apoptosis antagonizing tran... | NM_019816.1 | 79.7% | 77.9% | (many diffs) |
| 5 | mouse | 56321 | Aatf | apoptosis antagonizing tran... | XM_006533789.3 | 76.8% | 76.6% | (many diffs) |
| 6 | mouse | 56321 | Aatf | apoptosis antagonizing tran... | XR_388498.3 | 63.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1746
- ORF length:
- 1680
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt ggggccgcag cccctggcgc tgcaactgga acagttgttg aacccgcgac 121 caagcgaggc ggaccctgaa gcggaccccg aggaagccac tgctgccagg gtgattgaca 181 ggtttgatga aggggaagat ggggaaggtg atttcctagt agtgggtagc attagaaaac 241 tggcatcagc ctccctcttg gacacggaca aaaggtattg cggcaaaacc acctctagaa 301 aagcatggaa tgaagaccat tgggagcaga ctctgccagg atcgtctgat gaggaaatat 361 ctgatgagga agggtctgga gatgaagatt cagagggact gggtctggag gaatatgatg 421 aggacgacct gggtgctgct gaggaacagg agtgtggtga tcacagggag agcaagaaga 481 gcagaagcca ctctgcaaaa acaccgggct tcagtgtcca gagtatcagt gactttgaga 541 aatttaccaa gggaatggat gaccttggga gcagtgagga ggaggaagac gaagagagtg 601 gcatggaaga aggggatgac gcggaagact cccaaggcga gagtgaggaa gacagggctg 661 gagatagaaa cagtgaggat gatggtgtgg tgatgacctt ctctagtgtc aaagtttctg 721 aggaagtgga gaaaggaaga gccgtgaaga accagatagc actgtgggac cagctcttgg 781 aaggaaggat caaactacaa aaagctctgt tgaccaccaa ccagcttcct caaccagatg 841 ttttcccatt gttcaaggac aaaggtggcc cagaattttc cagtgccctg aaaaatagtc 901 acaaggcact taaagcattg ttgaggtcat tggtaggtct tcaggaagag ttgcttttcc 961 agtacccaga cactagatat ctagtagatg ggacaaagcc caatgcggga agtgaggaga 1021 tttctagtga agatgatgag ctggtagaag agaagaagca gcaacgaaga agggtccctg 1081 caaagaggaa gctggagatg gaggactatc ccagcttcat ggcaaagcgc tttgccgact 1141 ttacagtcta caggaaccgc acacttcaga aatggcacga taagaccaaa ctggcttctg 1201 gaaaactggg gaagggtttt ggtgcctttg aacgctcaat cttgactcag atcgaccata 1261 ttctgatgga caaagagaga ttacttcgaa ggacacagac caagcgctct gtctatcgag 1321 ttcttggcaa acctgagcca gcagctcagc ctgtcccaga gagtttgcca ggggaaccgg 1381 agatccttcc tcaagcccct gctaatgctc atctgaagga cttGGATGAA GAAATCTTTG 1441 ATGATGATGA CTTTTACCAC CAGCTCCTTC GAGAACTCAT AGAACGGAAG ACCAGCTCCT 1501 TGGATCCCAA CGATCAGGTG GCCATGGGAA GGCAGTGGCT TGCAATCCAG AAGTTACGAA 1561 GCAAAATCCA CAAAAAAGTA GATAGGAAAG CCAGCAAAGG CAGGAAACTT CGGTTTCATG 1621 TCCTTAGCAA GCTACTGAGT TTCATGGCAC CTATTGACCA TACTACAATG AATGATGATG 1681 CCAGGACAGA ACTGTACCGC TCTCTTTTTG GCCAGCTCCA CCCTCCCGAC GAAGGCCACG 1741 GGGATTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1801 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1861 CTTGGCTTTA TATATCTTGT GGAAAGGACG ATCCCAACAA TTTTAATTGT CCACCACGCG 1921 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt