Construct: ORF TRCN0000468404
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007229.1_s317c1
- Derived from:
- ccsbBroadEn_03131
- DNA Barcode:
- CTGAGAATCGAGCATCCTCACCCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TLX3 (30012)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468404
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 30012 | TLX3 | T cell leukemia homeobox 3 | NM_021025.4 | 100% | 100% | |
| 2 | mouse | 27140 | Tlx3 | T cell leukemia, homeobox 3 | NM_019916.2 | 95.1% | 99.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 939
- ORF length:
- 873
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ggcgcccgcc agcgcgcaga ccccgcaccc gcacgagccc atcagcttcg 121 gcatcgacca gatccttaac agcccggacc aggacagcgc acccgccccg cggggccccg 181 acggcgccag ctacctggga gggccccccg ggggccgtcc gggcgccaca tacccgtctc 241 tgcccgcctc ctttgcgggc ctcggcgcgc ccttcgagga cgcgggatct tacagtgtga 301 acctgagcct agcgcccgca ggcgtgatcc gggtgccggc gcacaggccg ctgcccgggg 361 ccgtgccacc gcctctgcca agcgcgctac ccgccatgcc ctccgtgccc acggtctcca 421 gccttggcgg tctcaatttc ccctggatgg agagcagccg ccgcttcgtg aaagaccgct 481 tcacagcggc ggccgcactc acgcccttca ccgtgacccg gcgcatcggc cacccctacc 541 agaaccggac gccgccCAAG CGTAAGAAGC CGCGCACGTC CTTTTCCCGG GTGCAGATCT 601 GCGAGCTGGA AAAGCGCTTC CATCGCCAGA AGTACCTGGC CTCTGCCGAG AGGGCGGCGC 661 TCGCCAAGTC CCTCAAAATG ACGGACGCGC AGGTCAAGAC CTGGTTCCAA AACCGGAGGA 721 CCAAGTGGCG GCGGCAGACG GCGGAGGAGC GGGAGGCGGA GCGGCAGCAG GCGAGCCGGC 781 TCATGCTGCA GCTGCAACAC GACGCCTTCC AAAAGAGCCT CAACGACTCC ATCCAGCCTG 841 ACCCGCTCTG TCTGCACAAC TCGTCACTCT TTGCTCTGCA GAATCTGCAG CCCTGGGAGG 901 AGGATAGTTC CAAGGTTCCC GCTGTCACCT CCCTGGTGTG CCCAACTTTC TTGTACAAAG 961 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1021 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1081 ACGACTGAGA ATCGAGCATC CTCACCCAAC GCGTTAAGTC gacaatcaac ctctggatta 1141 caaaatttgt gaaagatt