Construct: ORF TRCN0000468411
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001686.1_s317c1
- Derived from:
- ccsbBroadEn_02271
- DNA Barcode:
- CCGTTCTCCAGATTTACGTCCACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LPGAT1 (9926)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468411
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9926 | LPGAT1 | lysophosphatidylglycerol ac... | NM_001320808.2 | 99.8% | 99.7% | 652_653delACinsCA |
| 2 | human | 9926 | LPGAT1 | lysophosphatidylglycerol ac... | NM_014873.3 | 99.8% | 99.7% | 652_653delACinsCA |
| 3 | human | 9926 | LPGAT1 | lysophosphatidylglycerol ac... | XM_011510228.3 | 99.8% | 99.7% | 652_653delACinsCA |
| 4 | human | 9926 | LPGAT1 | lysophosphatidylglycerol ac... | XM_011510229.3 | 99.8% | 99.7% | 652_653delACinsCA |
| 5 | human | 9926 | LPGAT1 | lysophosphatidylglycerol ac... | XM_024451298.1 | 99.8% | 99.7% | 652_653delACinsCA |
| 6 | human | 9926 | LPGAT1 | lysophosphatidylglycerol ac... | XM_005273362.3 | 91.6% | 91.5% | 1_99del;751_752delACinsCA |
| 7 | human | 9926 | LPGAT1 | lysophosphatidylglycerol ac... | XM_017003004.1 | 82.9% | 79.6% | (many diffs) |
| 8 | mouse | 226856 | Lpgat1 | lysophosphatidylglycerol ac... | NM_172266.3 | 89.3% | 89.7% | (many diffs) |
| 9 | mouse | 226856 | Lpgat1 | lysophosphatidylglycerol ac... | XM_006497155.3 | 89.3% | 89.7% | (many diffs) |
| 10 | mouse | 226856 | Lpgat1 | lysophosphatidylglycerol ac... | XM_006497156.3 | 89.3% | 89.7% | (many diffs) |
| 11 | mouse | 226856 | Lpgat1 | lysophosphatidylglycerol ac... | NM_001134829.1 | 80.8% | 81.1% | (many diffs) |
| 12 | mouse | 226856 | Lpgat1 | lysophosphatidylglycerol ac... | XM_006497153.3 | 80.8% | 81.1% | (many diffs) |
| 13 | mouse | 226856 | Lpgat1 | lysophosphatidylglycerol ac... | XM_017320202.1 | 72.1% | 70.8% | (many diffs) |
| 14 | mouse | 226856 | Lpgat1 | lysophosphatidylglycerol ac... | XM_006497157.3 | 70.3% | 70.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1176
- ORF length:
- 1110
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tataactttg gaagaagctc cgtggctggg ctggctcttg gtgaaagcac 121 tgatgaggtt tgccttcatg gtcgtcaaca acctggttgc tattccatcc tacatctgct 181 atgtaattat acttcagccc cttcgagtgc tggacagtaa gcggttctgg tatatcgaag 241 gaatcatgta taaatggctt ttaggaatgg tagcttcctg gggatggtat gctggatata 301 cagtgatgga atggggagaa gatattaaag cagtttcaaa agatgaagca gtgatgttgg 361 tgaatcatca ggcaacagga gatgtgtgca cactgatgat gtgcctccag gacaaaggac 421 tggttgttgc tcagatgatg tggttgatgg atcatatttt taagtacaca aactttggaa 481 ttgtttctct agttcatgga gacttcttta taagacaggg aagatcttat cgtgaccaac 541 agctgctgct tctcaagaag cacttagaaa ataattacag gagcagagat cgaaaatgga 601 ttgttttgtt tccagaaggg ggcttcctca ggaagaggcg agaaacaagt caggcatttg 661 ccaagaaaaa taacttgcca tttcttacaa atgttactct gccaaggtct ggggcaCAAA 721 AAATTATTTT GAATGCACTT GTAGCACAAC AGAAAAATGG AAGTCCAGCA GGAGGAGATG 781 CTAAAGAATT AGACAGCAAA TCAAAAGGCC TCCAGTGGAT AATAGATACA ACGATAGCTT 841 ATCCCAAAGC TGAACCTATA GATATTCAAA CCTGGATCCT TGGATACAGG AAACCAACAG 901 TCACACATGT ACATTACAGG ATCTTTCCAA TTAAAGATGT ACCCCTGGAG ACTGATGACC 961 TTACCACTTG GCTCTATCAG CGGTTTGTTG AAAAAGAAGA CCTCTTATCA CATTTTTATG 1021 AAACAGGAGC TTTTCCACCT TCCAAGGGCC ATAAGGAAGC TGTTTCCAGG GAGATGACCC 1081 TCAGCAACTT GTGGATATTT CTCATACAGT CTTTTGCATT TTTGTCAGGC TATATGTGGT 1141 ACAACATCAT TCAGTATTTT TACCATTGCC TGTTTTACCC AACTTTCTTG TACAAAGTGG 1201 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1261 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1321 ACCGTTCTCC AGATTTACGT CCACGACGCG TTAAGTCgac aatcaacctc tggattacaa 1381 aatttgtgaa agatt