Transcript: Mouse NM_001134829.1

Mus musculus lysophosphatidylglycerol acyltransferase 1 (Lpgat1), transcript variant 1, mRNA.

Source:
NCBI, updated 2017-05-21
Taxon:
Mus musculus (mouse)
Gene:
Lpgat1 (226856)
Length:
7069
CDS:
114..1343

Additional Resources:

NCBI RefSeq record:
NM_001134829.1
NBCI Gene record:
Lpgat1 (226856)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001134829.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000125609 GCTACATAGGAGTTCAGACTT pLKO.1 1360 3UTR 100% 4.950 6.930 N Lpgat1 n/a
2 TRCN0000323855 GCTACATAGGAGTTCAGACTT pLKO_005 1360 3UTR 100% 4.950 6.930 N Lpgat1 n/a
3 TRCN0000125613 CGCTAAAGATGAAGCAGTGAT pLKO.1 500 CDS 100% 4.950 3.960 N Lpgat1 n/a
4 TRCN0000125610 CCATCCTACATCTGCTATGTA pLKO.1 330 CDS 100% 0.563 0.450 N Lpgat1 n/a
5 TRCN0000323921 CCATCCTACATCTGCTATGTA pLKO_005 330 CDS 100% 0.563 0.450 N Lpgat1 n/a
6 TRCN0000125611 CCTCAGCAACATGTGGATATT pLKO.1 1244 CDS 100% 13.200 9.240 N Lpgat1 n/a
7 TRCN0000323923 CCTCAGCAACATGTGGATATT pLKO_005 1244 CDS 100% 13.200 9.240 N Lpgat1 n/a
8 TRCN0000125612 CTAGAGGCTTAGAATGCAAAT pLKO.1 946 CDS 100% 10.800 7.560 N Lpgat1 n/a
9 TRCN0000323853 CTAGAGGCTTAGAATGCAAAT pLKO_005 946 CDS 100% 10.800 7.560 N Lpgat1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001134829.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02271 pDONR223 100% 81% 81.4% None (many diffs) n/a
2 ccsbBroad304_02271 pLX_304 0% 81% 81.4% V5 (many diffs) n/a
3 TRCN0000468411 CCGTTCTCCAGATTTACGTCCACG pLX_317 41.5% 80.8% 81.1% V5 (many diffs) n/a
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