Construct: ORF TRCN0000468419
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004274.1_s317c1
- Derived from:
- ccsbBroadEn_09550
- DNA Barcode:
- ATCCGTGTCATGTACCAAGTCGTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- UGT3A1 (133688)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468419
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 133688 | UGT3A1 | UDP glycosyltransferase fam... | NM_001171873.2 | 99.8% | 99.6% | 751T>C |
| 2 | human | 133688 | UGT3A1 | UDP glycosyltransferase fam... | XM_005248243.4 | 53% | 49.4% | (many diffs) |
| 3 | human | 133688 | UGT3A1 | UDP glycosyltransferase fam... | XM_011513959.2 | 53% | 49.4% | (many diffs) |
| 4 | human | 133688 | UGT3A1 | UDP glycosyltransferase fam... | XM_011513958.2 | 49.7% | 45.8% | (many diffs) |
| 5 | human | 133688 | UGT3A1 | UDP glycosyltransferase fam... | XM_011513957.2 | 49% | 44.1% | (many diffs) |
| 6 | human | 133688 | UGT3A1 | UDP glycosyltransferase fam... | NM_152404.4 | 47.5% | 44.3% | (many diffs) |
| 7 | human | 133688 | UGT3A1 | UDP glycosyltransferase fam... | XR_001741997.1 | 31.5% | (many diffs) | |
| 8 | human | 133688 | UGT3A1 | UDP glycosyltransferase fam... | XR_001741998.1 | 29.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 822
- ORF length:
- 756
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct tcatcagagt ggaaagtttt tgatcccaga tattaaagag gaggaaaaat 121 cataccaagt tatcaggtgg ttttcacctg aagatcatca aaaaagaatt aagaagcatt 181 ttgatagcta catagaaaca gcattggatg gcagaaaaga atctgaagcc cttgtaaagc 241 taatggaaat atttgggact caatgtagtt atttgctaag cagaaaggat ataatggatt 301 ccttaaagaa tgagaactat gatctggtat ttgttgaagc atttgatttc tgttctttcc 361 TGATTGCTGA GAAGCTTGTG AAACCATTTG TGGCCATTCT TCCCACCACA TTCGGCTCTT 421 TGGATTTTGG GCTACCAAGC CCCTTGTCTT ATGTTCCAGT ATTCCCTTCC TTGCTGACTG 481 ATCACATGGA CTTCTGGGGC CGAGTGAAGA ATTTTCTGAT GTTCTTTAGT TTCTCCAGGA 541 GCCAATGGGA CATGCAGTCT ACATTTGACA ACACCATCAA GGAGCATTTC CCAGAAGGCT 601 CTAGGCCAGT TTTGTCTCAT CTTCTACTGA AAGCAGAGTT GTGGTTTGTT AACTCTGATT 661 TTGCCTTTGA TTTTGCCCGG CCCCTGCTTC CCAACACTGT TTATATTGGA GGCTTGATGG 721 AAAAACCTAT TAAACCAGTA CCACAAAATG GGCAACCAGC TCTCTTCACC ACCCCCAGCT 781 TATTCTCCTC TGGAGTGTAT CCTGAACCAC TGAGACGGCT TTGCCCAACT TTCTTGTACA 841 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 901 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 961 AGGACGAATC CGTGTCATGT ACCAAGTCGT TACGCGTTAA GTCgacaatc aacctctgga 1021 ttacaaaatt tgtgaaagat t