Transcript: Human XR_001741997.1

PREDICTED: Homo sapiens UDP glycosyltransferase family 3 member A1 (UGT3A1), transcript variant X5, misc_RNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
UGT3A1 (133688)
Length:
2370
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_001741997.1
NBCI Gene record:
UGT3A1 (133688)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XR_001741997.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000150035 CACACTGACACTTACAATGAA pLKO.1 1411 3UTR 100% 5.625 4.500 N UGT3A1 n/a
2 TRCN0000150012 CCCTTGTAAAGCTAATGGAAA pLKO.1 483 3UTR 100% 4.950 3.960 N UGT3A1 n/a
3 TRCN0000435337 TGGGACATGCAGTCTACATTT pLKO_005 800 3UTR 100% 13.200 9.240 N UGT3A1 n/a
4 TRCN0000423257 TGGTGGGCAGAACAGCGTAAT pLKO_005 1264 3UTR 100% 10.800 7.560 N UGT3A1 n/a
5 TRCN0000147673 GATGTTCATTTGGCCACAAAT pLKO.1 1172 3UTR 100% 1.320 0.924 N UGT3A1 n/a
6 TRCN0000150098 CCTTGTCTTATGTTCCAGTAT pLKO.1 696 3UTR 100% 4.950 2.475 Y UGT3A1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_001741997.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04885 pDONR223 100% 60.7% None (many diffs) n/a
2 ccsbBroad304_04885 pLX_304 0% 60.7% V5 (many diffs) n/a
3 TRCN0000471431 GCAGTGCGTTCTTTAACGATTGAA pLX_317 28.1% 60.7% V5 (many diffs) n/a
4 ccsbBroadEn_09550 pDONR223 100% 31.5% None (many diffs) n/a
5 ccsbBroad304_09550 pLX_304 0% 31.5% V5 (many diffs) n/a
6 TRCN0000468419 ATCCGTGTCATGTACCAAGTCGTT pLX_317 60.8% 31.5% V5 (many diffs) n/a
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