Construct: ORF TRCN0000468423
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002578.1_s317c1
- Derived from:
- ccsbBroadEn_05188
- DNA Barcode:
- AGATGTTTGCTGATACTGACCGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ANKRD23 (200539)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468423
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 200539 | ANKRD23 | ankyrin repeat domain 23 | NM_144994.8 | 100% | 100% | |
| 2 | mouse | 78321 | Ankrd23 | ankyrin repeat domain 23 | NM_153502.4 | 86% | 82.3% | (many diffs) |
| 3 | mouse | 78321 | Ankrd23 | ankyrin repeat domain 23 | NM_001310521.1 | 54.5% | 54% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 984
- ORF length:
- 915
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggacttcatc agcattcagc agttggtaag tggagaaaga gttgaaggga 121 aagtgttggg atttggacat ggagttcctg accctggagc ctggcctagt gactggagga 181 ggggccccca agaggctgtg gcccgggaga agctgaaatt ggaagaagag aagaagaaga 241 aacttgaaag atttaacagt accagattta atctggataa cctggctgac ttggaaaact 301 tggttcaaag acggaaaaag cgactgagac acagagtccc ccccaggaaa cctgagcccc 361 tggttaagcc gcagtcccag gcccaggtgg agcctgtggg cctggagatg ttcctgaagg 421 cagctgctga gaaccaggag tacctgattg acaagtactt gacagacgga ggggacccca 481 atgcccatga caagctccac cgcaccgcct tgcactgggc ctgtctgaag ggtcacagcc 541 agctggtgaa caagctgctg gtggcaggtg ccacagtgga cgcgcgagac ttgctggaca 601 ggacacctgt gttctgggcc tgccgcggag gacatctggt catccTCAAA CAGCTGCTTA 661 ACCAGGGAGC CCGGGTCAAT GCCCGGGACA AGATCGGGAG CACCCCCCTG CACGTGGCAG 721 TGCGCACCCG GCACCCCGAC TGCCTGGAGC ACCTCATCGA GTGTGGCGCC CACCTGAACG 781 CACAGGATAA GGAAGGGGAC ACGGCTCTGC ACGAGGCCGT GCGGCACGGC AGCTACAAAG 841 CCATGAAGCT ACTGCTGCTC TATGGGGCCG AGCTGGGGGT GCGGAACGCG GCCTCCGTGA 901 CCCCGGTGCA GCTGGCTCGA GACTGGCAGC GCGGCATCCG GGAGGCCCTG CAGGCCCACG 961 TGGCGCATCC CCGCACCCGG TGCTTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1021 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1081 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA GATGTTTGCT 1141 GATACTGACC GTCACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1201 att