Construct: ORF TRCN0000468434
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017032.1_s317c1
- Derived from:
- ccsbBroadEn_02167
- DNA Barcode:
- GTTTTATTCCGCCCGTACGACTTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ITM2A (9452)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468434
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9452 | ITM2A | integral membrane protein 2A | NM_004867.5 | 100% | 100% | |
| 2 | human | 9452 | ITM2A | integral membrane protein 2A | NM_001171581.1 | 83.2% | 83.2% | 107_108ins132 |
| 3 | mouse | 16431 | Itm2a | integral membrane protein 2A | NM_008409.2 | 89.6% | 95% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 855
- ORF length:
- 789
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt gaaaatcgcc ttcaataccc ctaccgccgt gcaaaaggag gaggcgcggc 121 aagacgtgga ggccctcctg agccgcacgg tcagaactca gatactgacc ggcaaggagc 181 tccgagttgc cacccaggaa aaagagggct cctctgggag atgtatgctt actctcttag 241 gcctttcatt catcttggca ggacttattg ttggtggagc ctgcatttac aagtacttca 301 tgcccaagag caccatttac cgtggagaga tgtgcttttt tgattctgag gatcctgcaa 361 attcccttcg tggaggagag ccTAACTTCC TGCCTGTGAC TGAGGAGGCT GACATTCGTG 421 AGGATGACAA CATTGCAATC ATTGATGTGC CTGTCCCCAG TTTCTCTGAT AGTGACCCTG 481 CAGCAATTAT TCATGACTTT GAAAAGGGAA TGACTGCTTA CCTGGACTTG TTGCTGGGGA 541 ACTGCTATCT GATGCCCCTC AATACTTCTA TTGTTATGCC TCCAAAAAAT CTGGTAGAGC 601 TCTTTGGCAA ACTGGCGAGT GGCAGATATC TGCCTCAAAC TTATGTGGTT CGAGAAGACC 661 TAGTTGCTGT GGAGGAAATT CGTGATGTTA GTAACCTTGG CATCTTTATT TACCAACTTT 721 GCAATAACAG AAAGTCCTTC CGCCTTCGTC GCAGAGACCT CTTGCTGGGT TTCAACAAAC 781 GTGCCATTGA TAAATGCTGG AAGATTAGAC ACTTCCCCAA CGAATTTATT GTTGAGACCA 841 AGATCTGTCA AGAGTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 901 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 961 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA GTTTTATTCC GCCCGTACGA 1021 CTTGACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt