Construct: ORF TRCN0000468441
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015517.1_s317c1
- Derived from:
- ccsbBroadEn_12924
- DNA Barcode:
- GATATATGGATTCGATGTGAGCGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SEC16B (89866)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468441
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 89866 | SEC16B | SEC16 homolog B, endoplasmi... | NM_001356499.2 | 99.7% | 99.6% | (many diffs) |
| 2 | human | 89866 | SEC16B | SEC16 homolog B, endoplasmi... | NM_033127.3 | 57.5% | 56.4% | (many diffs) |
| 3 | human | 111240474 | CRYZL2P-SEC16B | CRYZL2P-SEC16B readthrough | NM_001356505.1 | 57.5% | 56.4% | (many diffs) |
| 4 | human | 111240474 | CRYZL2P-SEC16B | CRYZL2P-SEC16B readthrough | NM_001356506.1 | 57.5% | 56.4% | (many diffs) |
| 5 | human | 111240474 | CRYZL2P-SEC16B | CRYZL2P-SEC16B readthrough | NR_151492.2 | 45.1% | (many diffs) | |
| 6 | human | 89866 | SEC16B | SEC16 homolog B, endoplasmi... | NM_001356500.1 | 25.6% | 24.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1941
- ORF length:
- 1875
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga actttgggct ccccagaggc tgccccagac acgagggaag gccacagcac 121 cctcaaagga tccagaccga gggtttcgga gagatggaca tcatcggcct gtccctcact 181 cttggcacaa tggagagagg tttcaccaat ggcaagacaa ccgtgggagc ccccagccac 241 agcaggagcc cagggcagac catcagcagc agccccatta tgcatccagg ccaggggact 301 ggcatcagcc tgtgtctgga gttgactatt acgaaggtgg ttatcgcaat cagttgtatt 361 caaggccagg ttatgagaat tcatatcaga gctatcagtc tcccacaatg agggaggaat 421 atgcttatgg aagttattac tatcatggac acccacagtg gctgcaggaa gaaagagtgc 481 caaggcaacg gagtccttat atctggcacg aagattaccg agagcaaaag taccttgatg 541 aacatcatta tgaaaaccag cacagtccat ttggaacaaa tagtgagacc cacttccaat 601 ctaacagtag gaacccttgt aaagacagcc ctgcttccaa ctctggacag gagtggccgg 661 gggagctgtt tccagggagc ctgcttgctg aggcccagaa aaataagccg tcattggcta 721 gtgagtccaa ccttctccag cagcgtgagt ctggtctcag ctccagcagc tatgagctca 781 gtcagtacat cagagatgcc ccggagcggg atgatccccc agcttcagca gcttggagtc 841 cagttcaggc tgaagatgtc tcctcagctg gtcccaaagc acccatgaag ttctacatcc 901 ctcatgttcc tgtgagtttc gggccaggag gtcagctggt gcgtgtaggt cccagctctc 961 ccactgacgg gcaagcagcc cttgttgaat tgcacagcat ggaggttatt cttaatgatt 1021 ccgaagagca agaggagatg agaagtttct caggaccctt gattagggaa gatgtacata 1081 aggtggatat tatgacgttt tgccagcaga aagcagctca gagctgcaaa tctgagacac 1141 tggggagcag agactcagct ctactgtggc agctcttggt tctcctttgt cgccagaatg 1201 ggtccatggt ggggtctgac atcgctgagc tgctaatgca agactgcaag aagctggaga 1261 agtacaagcg gcagccccct gtggccaacc tcatcaacct gaccgatgag gactggccag 1321 tgctgagctc tgggaccccg aacctgctca cgggagagat cccccccagt gtggagacac 1381 ctgcgcagat cgtggagaaa ttcactaggc tgctctacta tggaaggaag aaggaagcct 1441 tggagtgggc catgaagaac cacttgtggg gccatgcttt gttcctgtcc agcaagatGG 1501 ACCCACAGAC CTACAGCTGG GTCATGAGTG GCTTCACCAG CACGCTGGCG CTCAATGACC 1561 CACTGCAGAC CCTCTTCCAG CTCATGTCGG GGAGGATTCC ACAGGCAGCC ACGTGTTGTG 1621 GAGAAAAGCA GTGGGGAGAC TGGAGGCCTC ACTTGGCTGT GATTCTGTCG AATCAGGCTG 1681 GGGACCCAGA GCTGTATCAG CGTGCGATTG TTGCCATTGG GGACACCCTG GCTGGGAAGG 1741 GGCTTGTGGA GGCAGCTCAC TTCTGCTATC TCATGGCTCA CGTGCCCTTT GGCCACTACA 1801 CCGTGAAGAC AGACCATCTG GTCTTGCTGG GCAGCAGCCA CAGGTATGCG ACCTGGGAGA 1861 AAGGAAACAG TAAGGACATT TTCCAAGGGA CTGTTTTAGC CCTTGTTGGG TTTTATGGAT 1921 CCAGTTTTCA TTTTCTCATG TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1981 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 2041 AAAGTATTTC GATTTCTTGG CCTTATATAT CTTGTGGAAA GGACGAGATA TATGGATTCG 2101 ATGTGAGCGT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt