Transcript: Human NM_001356499.2

Homo sapiens SEC16 homolog B, endoplasmic reticulum export factor (SEC16B), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-08-08
Taxon:
Homo sapiens (human)
Gene:
SEC16B (89866)
Length:
2976
CDS:
114..1991

Additional Resources:

NCBI RefSeq record:
NM_001356499.2
NBCI Gene record:
SEC16B (89866)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001356499.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000148895 CCCACTTCCAATCTAACAGTA pLKO.1 637 CDS 100% 4.950 2.475 Y SEC16B n/a
2 TRCN0000130820 GCTGCTCTACTATGGAAGGAA pLKO.1 1457 CDS 100% 3.000 1.500 Y SEC16B n/a
3 TRCN0000130892 GAGAGGTTTCACCAATGGCAA pLKO.1 243 CDS 100% 2.640 1.320 Y SEC16B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001356499.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12924 pDONR223 100% 99.7% 99.6% None (many diffs) n/a
2 ccsbBroad304_12924 pLX_304 0% 99.7% 99.6% V5 (many diffs) n/a
3 TRCN0000468441 GATATATGGATTCGATGTGAGCGT pLX_317 23.9% 99.7% 99.6% V5 (many diffs) n/a
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