Construct: ORF TRCN0000468442
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017688.1_s317c1
- Derived from:
- ccsbBroadEn_08721
- DNA Barcode:
- TGATGCCCGGGAGCAGTTAATGAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- JPH3 (57338)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468442
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57338 | JPH3 | junctophilin 3 | NM_020655.4 | 99.8% | 99.5% | 1127T>C;1414C>A;1934C>T |
| 2 | human | 57338 | JPH3 | junctophilin 3 | NR_073379.2 | 69.8% | (many diffs) | |
| 3 | human | 57338 | JPH3 | junctophilin 3 | NM_001271604.3 | 22.4% | 17.6% | (many diffs) |
| 4 | human | 57338 | JPH3 | junctophilin 3 | NM_001271605.2 | 18.8% | 17.4% | (many diffs) |
| 5 | mouse | 57340 | Jph3 | junctophilin 3 | NM_020605.3 | 87.8% | 90.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2310
- ORF length:
- 2244
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc cagtgggggc aggtttaatt ttgacgacgg agggtcctac tgtggaggct 121 gggaggacgg caaggcgcac ggccatggcg tctgcaccgg ccccaagggc caaggcgaat 181 acaccggctc gtggagccac ggcttcgagg tgctgggcgt ctacacctgg cccagcggca 241 acacgtacca gggcacctgg gcgcagggca agcgccacgg catcggcctg gagagcaagg 301 ggaagtgggt gtacaagggc gagtggacgc acggattcaa ggggcgctac ggggtgcggg 361 agtgcgcggg caacggggcc aaatacgaag ggacctggag caacgggctg caggacggct 421 acgggaccga gacctactcg gacggaggga cctaccaggg ccagtgggtc ggtggcatgc 481 gccagggcta cggcgtccgg cagagcgtcc cgtatggcat ggccgcggtc atccgctcac 541 ccctgaggac gtccatcaac tccctgcgca gcgagcacac caacggcacg gcgctgcatc 601 ccgacgcctc tccggcggtg gccggcagcc cggccgtgtc ccgcgggggc ttcgtgctcg 661 tggcccacag tgactccgag atcctcaaga gcaagaagaa ggggctgttt cggcgctcgc 721 tgctgagtgg gctgaagctg cgcaagtcgg agtccaagag cagcctggcc agccaacgca 781 gcaagcagag ctcctttcgc agcgaggcgg gcatgagcac cgtcagctcc acggccagcg 841 acatccactc caccatcagc ctgggcgagg ctgaggccga gctggcggtc atcgaggacg 901 acatcgacgc caccaccacc gagacctacg tgggcgagtg gaagaacgac aaacgctccg 961 gcttcggcgt gagccagcgc tcggacgggc tcaagtacga gggcgagtgg gccagcaacc 1021 ggcgccatgg ctacggctgc atgaccttcc cggacggcac caaggaggag ggcaagtaca 1081 agcagaacat cctcgtcggc ggcaagcgca agaacctcat ccccctgcgg gccagcaaga 1141 tccgcgagaa ggtggaccgc gccgttgagg ccgctgagcg ggccgccacc accgccaagc 1201 agaaggctga gatcgcggct tccaggacct cccactctcg ggcaaaggcc gaggcagccc 1261 tcacagcagc tcagaaagcc caggaggagg cgcggatcgc caggatcact gccaaagagt 1321 tctccccttc cttccagcac cgggaaaacg ggctggagta ccagaggccg aagcgtcaga 1381 cctcctgtga cgacatcgag gtgctgtcca ccgggacacc cctgcagcag gagagccccg 1441 agctgtaccg caagggcacc actccctccg acctgaccac cgacgacagc cccctgcaga 1501 gcttccccac cagccccgcg gccaccccgc cgcccgcgcc cgccgccagg aacaaggtcg 1561 cccacttctc gaggcaggtg tcggtggacg aggagcgggg cggggacatc cagatgctcc 1621 tggagggccg ggccggggac tgcgcccgca gcagctgggg cgaggagcag gccgggggct 1681 ccaggggtgt ccgcagcggt gccctgcgcg gcggcctgct cgtggatgac ttccgcaccc 1741 gaggttcggg ccgcaagcag cccgggaacc ccaagccgcg ggagcggcgg acggagtcac 1801 cccccgtgtt cacgtggact tcccaccacc gggccagcaa ccacagcccc ggaggctcca 1861 ggctgctgga gctgcaggag gagaagctga gcaactaccg gatggagatg aaacccttgc 1921 tgaggatGGA GACGCATCCC CAGAAAAGAC GCTACAGCAA GGGCGGCGCC TGCCGGGGCT 1981 TGGGGGACGA CCACCGCCTC GAGGACCGGG GCTTCGGGGT GCAGAGACTG CGGTCCAAGG 2041 CCCAGAACAA GGAGAACTTC AGGCCGGCCT CCTCCGCGGA GCCCGCCGTG CAGAAACTGG 2101 CGAGCCTGCG GCTGGGCGGG GCCGAGCCCC GGTTGCTGCG TTGGGACTTG ACCTTCTCCC 2161 CGCCCCAGAA ATCCTTGCCT GTCGCTCTAG AGTCCGACGA GGAGAATGGG GATGAGCTCA 2221 AGTCCAGTAC GGGCTCAGCG CCTATCCTGG TGGTCATGGT GATCTTGCTC AACATCGGAG 2281 TCGCCATTCT GTTTATTAAC TTTTTCATCT GCCCAACTTT CTTGTACAAA GTGGTTGATA 2341 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 2401 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGATGATG 2461 CCCGGGAGCA GTTAATGATA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 2521 tgaaagatt