Construct: ORF TRCN0000468449
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004580.1_s317c1
- Derived from:
- ccsbBroadEn_09920
- DNA Barcode:
- GACCCTACTTGATAGCTATCTTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GCSAM (257144)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468449
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 257144 | GCSAM | germinal center associated ... | NM_152785.5 | 99.8% | 99.4% | 13C>G |
| 2 | human | 257144 | GCSAM | germinal center associated ... | XM_024453437.1 | 99.2% | 98.8% | 13C>G;217_219delCAG |
| 3 | human | 257144 | GCSAM | germinal center associated ... | NM_001190259.2 | 98.7% | 98.3% | 13C>G;27_32delCAGTTT |
| 4 | human | 257144 | GCSAM | germinal center associated ... | XM_011512627.3 | 98.1% | 97.7% | 13C>G;27_32delCAGTTT;223_225delCAG |
| 5 | human | 257144 | GCSAM | germinal center associated ... | XM_024453438.1 | 95.7% | 94.4% | (many diffs) |
| 6 | human | 257144 | GCSAM | germinal center associated ... | NM_001190260.2 | 91.3% | 91% | 13C>G;97_98ins45 |
| 7 | human | 257144 | GCSAM | germinal center associated ... | XM_005247272.3 | 90.4% | 90.4% | 0_1ins51 |
| 8 | human | 257144 | GCSAM | germinal center associated ... | XM_024453439.1 | 90.4% | 90.4% | 0_1ins51 |
| 9 | human | 257144 | GCSAM | germinal center associated ... | XM_011512629.2 | 89.9% | 89.9% | 0_1ins51;166_168delCAG |
| 10 | human | 257144 | GCSAM | germinal center associated ... | XM_011512630.2 | 89.9% | 89.9% | 0_1ins51;166_168delCAG |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 600
- ORF length:
- 534
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg aaattctgtg ctgagagaaa acaggcggca gcagaacact caagagatgc 121 cttggaatgt gagaatgcaa agccccaaac agagaacatc cagatgctgg gatcaccata 181 tcgctgaagg gtgtttctgc cttccatgga aaaaaatact catttttgaa aagaggcaag 241 attcccaaaa cgaaaatgaa agaatgtcat ctactcccat ccaggacaat gttgaccaga 301 cctactcaga ggagctgtgc tataccctca tcaatcatcg ggttctctgt acaaggccat 361 cagggaactc tgctgaagag tactatgaga atgttccctg caaagctgag agacccagag 421 agtCCTTGGG AGGAACTGAG ACTGAGTATT CACTTCTACA TATGCCTTCT ACAGACCCCA 481 GGCATGCCCG ATCCCCAGAA GATGAATATG AACTTCTCAT GCCTCACAGA ATCTCCTCTC 541 ACTTTCTGCA ACAGCCACGT CCACTTATGG CCCCTTCTGA GACTCAGTTT TCCCATTTAT 601 ACCCAACTCT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 661 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 721 TTTATATATC TTGTGGAAAG GACGAGACCC TACTTGATAG CTATCTTACA CGCGTTAAGT 781 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt