Construct: ORF TRCN0000468483
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012519.1_s317c1
- Derived from:
- BRDN0000395435
- DNA Barcode:
- AAGCTCTTCGCTAGCGTTAGCACT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HNRNPU (3192)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468483
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3192 | HNRNPU | heterogeneous nuclear ribon... | NM_031844.3 | 87.3% | 87.1% | 1_309del;330_331delCGinsAA;1764_1765delCCinsGG |
| 2 | human | 3192 | HNRNPU | heterogeneous nuclear ribon... | NM_004501.3 | 85% | 84.8% | (many diffs) |
| 3 | mouse | 51810 | Hnrnpu | heterogeneous nuclear ribon... | NM_016805.3 | 79.3% | 83.6% | (many diffs) |
| 4 | mouse | 51810 | Hnrnpu | heterogeneous nuclear ribon... | NR_149827.1 | 38.9% | (many diffs) | |
| 5 | mouse | 51810 | Hnrnpu | heterogeneous nuclear ribon... | XR_003956224.1 | 32.4% | (many diffs) | |
| 6 | mouse | 51810 | Hnrnpu | heterogeneous nuclear ribon... | XR_003956223.1 | 25.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2232
- ORF length:
- 2166
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gctaggagag gagaaaaggg ccgcgggggc ggccgactcg ggcccgatgg 121 aggaggagga ggccgcctcg gaagacgaga acggcgacga tcagggtttc caggaagggg 181 aagatgagct cggggacgaa gaggaaggcg cgggcgacga gaacgggcac ggggagcagc 241 agcctcaacc gccggcgacg cagcagcaac agccccaaca gcagcgcggg gccgccaagg 301 aggccgcggg gaagagcagc ggccccacct cgctgttcgc ggtgacggtg gcgccgcccg 361 gggcgaggca gggccagcag caggcgggag gtaagaagaa ggcggaaggc ggcggaggcg 421 gcggtcgccc cggggctccg gcggcggggg acggcaaaac agaacagaaa ggcggagata 481 aaaagagggg tgttaaaaga ccacgagaag atcatggccg tggatatttt gagtacattg 541 aagagaacaa gtatagcaga gccaaatctc ctcagccacc tgttgaagaa gaagatgaac 601 acttcgatga cacagtggtt tgtcttgata cttataattg tgatctacat tttaaaatat 661 caagagatcg tctcagtgct tcttccctta caatggagag ttttgctttt ctttgggctg 721 gaggaagagc atcctatggt gtgtcaaaag gcaaagtgtg ttttgagatg aaggttacag 781 agaagatccc agtaaggcat ttatatacaa aagatattga catacatgaa gttcgtattg 841 gctggtcact aactacaagt ggaatgttac ttggtgaaga agaattttct tatgggtatt 901 ctctaaaagg aataaaaaca tgcaactgtg agactgaaga ttatggagaa aagtttgatg 961 aaaatgatgt gattacatgt tttgctaact ttgaaagtga tgaagtagaa ctctcgtatg 1021 ctaagaatgg acaagatctt ggcgttgcct tcaaaatcag taaggaagtt cttgctggac 1081 ggccactgtt cccgcatgtt ctctgccaca actgtgcagt tgaatttaat tttggtcaga 1141 aggaaaagcc atattttcca atacctgaag agtatacttt catccagaac gtccccttag 1201 aggatcgagt tagaggacca aaggggcctg aagagaagaa agattgtgaa gttgtgatga 1261 tgattggctt gccaggagct ggaaaaacta cctgggttac taaacatgca gcagaaaatc 1321 cagggaaata taacattctt ggcacaaata ctattatgga taagatgatg gtggcaggtt 1381 ttaagaagca aatggcagat actggaaaac tgaacacact gttgcagaga gccccccagt 1441 gtcttgggaa atttattgag attgctgccc gaaagaagcg aaattttatt ctggatcaga 1501 caaatgtgtc tgctgctgcg gagaggagaa aaatgtgcct gtttgcaggc ttccagcgaa 1561 aagctgttgt agtttgccca aaagatgaag actataagca aagaacacag aagaaagcag 1621 aagtagaggg gaaagaccta ccagaacatg cggtcctcaa aatgaaagga aactttaccc 1681 tcccagaggt agctgagtgc tttgatgaaa taacctatgt tgaacttcag aaggaagaag 1741 cccaaaaact cttggagcaa tataaggaag aaagcaaaaa ggctcttcca ccagaaaaga 1801 aacagaacac tggctcaaag aaaagcaata aaaataagag tggcaagaac cagtttaaca 1861 gaggtggtgg ccatagagga cgtgGAGGAT TCAATATGCG TGGTGGAAAT TTCAGAGGAG 1921 GAGCCCCTGG GAATCGTGGC GGATATAATA GGAGGGGCAA CATGCCACAG AGAGGTGGTG 1981 GCGGTGGAGG AAGTGGTGGA ATCGGCTATC CATACCCTCG TGCCCCTGTT TTTCCTGGCC 2041 GTGGTAGTTA CTCAAACAGA GGGAACTACA ACAGAGGTGG AATGCCCAAC AGAGGGAACT 2101 ACAACCAGAA CTTCAGAGGA CGAGGAAACA ATCGTGGCTA CAAAAATCAA TCTCAGGGCT 2161 ACAACCAGTG GCAGCAGGGT CAATTCTGGG GTCAGAAGCC ATGGAGTCAG CATTATCACC 2221 AAGGATATTA TTGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 2281 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 2341 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAAAG CTCTTCGCTA GCGTTAGCAC 2401 TACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t