Construct: ORF TRCN0000468485
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018257.1_s317c1
- Derived from:
- ccsbBroadEn_05638
- DNA Barcode:
- CGCGGGCGGCACTGTACAGTGTGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NCMAP (400746)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468485
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 400746 | NCMAP | non-compact myelin associat... | NM_001010980.5 | 100% | 100% | |
| 2 | human | 400746 | NCMAP | non-compact myelin associat... | XM_005245872.3 | 100% | 100% | |
| 3 | human | 400746 | NCMAP | non-compact myelin associat... | XM_011541463.2 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 93
- ORF end:
- 399
- ORF length:
- 306
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA CCATGACCAC AGCCACCCCT CTGGGGGATA 121 CCACCTTCTT CTCACTGAAC ATGACCACCA GGGGAGAAGA CTTCCTGTAT AAGAGTTCTG 181 GAGCCATTGT TGCTGCCGTT GTGGTGGTTG TCATCATCAT CTTCACCGTG GTTCTGATCC 241 TGCTGAAGAT GTACAACAGG AAAATGAGGA CGAGGCGGGA ACTAGAGCCC AAGGGCCCCA 301 AGCCAACCGC CCCTTCTGCC GTGGGCCCAA ACAGCAACGG CAGCCAACAC CCAGCAACTG 361 TGACCTTCAG TCCTGTTGAC GTCCAGGTGG AGACGCGATT GCCAACTTTC TTGTACAAAG 421 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 481 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 541 ACGACGCGGG CGGCACTGTA CAGTGTGGAC GCGTTAAGTC gacaatcaac ctctggatta 601 caaaatttgt gaaagatt