Construct: ORF TRCN0000468486
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004935.1_s317c1
- Derived from:
- ccsbBroadEn_07393
- DNA Barcode:
- AGTCGGTGAACTTCCGATAGCCGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GLP2R (9340)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468486
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9340 | GLP2R | glucagon like peptide 2 rec... | NM_004246.3 | 99.8% | 99.8% | 1408G>A;1512T>C |
| 2 | human | 9340 | GLP2R | glucagon like peptide 2 rec... | XM_017025339.1 | 98.8% | 98.7% | 381_398del;1426G>A;1530T>C |
| 3 | human | 9340 | GLP2R | glucagon like peptide 2 rec... | XM_011524077.3 | 95.5% | 95.5% | 380_454del;1483G>A;1587T>C |
| 4 | human | 9340 | GLP2R | glucagon like peptide 2 rec... | XM_017025340.1 | 87.2% | 82.2% | (many diffs) |
| 5 | human | 9340 | GLP2R | glucagon like peptide 2 rec... | XM_017025341.1 | 80.8% | 80.8% | 189_190ins315;1093G>A;1197T>C |
| 6 | human | 9340 | GLP2R | glucagon like peptide 2 rec... | XM_005256861.3 | 72.2% | 69.5% | (many diffs) |
| 7 | mouse | 93896 | Glp2r | glucagon-like peptide 2 rec... | NM_175681.3 | 77.6% | 74.8% | (many diffs) |
| 8 | mouse | 93896 | Glp2r | glucagon-like peptide 2 rec... | XM_006534561.3 | 76.2% | 73.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1728
- ORF length:
- 1659
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaagctggga tcgagcaggg cagggcctgg gagaggaagc gcgggactcc 121 tgcctggcgt ccacgagctg cccatgggca tccctgcccc ctgggggacc agtcctctct 181 ccttccacag gaagtgctct ctctgggccc ctgggaggcc cttcctcact ctggtcctgc 241 tggtttccat caagcaagtt acaggatccc tccttgagga aacgactcgg aagtgggctc 301 agtacaaaca ggcatgtctg agagacttac tcaaggaacc ttctggcata ttttgtaacg 361 ggacatttga tcagtacgtg tgttggcctc attcttctcc tggaaatgtc tctgtaccct 421 gcccttcata cttaccttgg tggagtgaag agagctcagg aagggcctac agacactgct 481 tggctcaggg gacttggcag acgatagaga acgccacgga tatttggcag gatgactccg 541 aatgctccga gaaccacagc ttcaagcaaa acgtggatcg ttatgccttg ctgtcaacct 601 tgcagctgat gtacaccgtg ggatactcct tctctcttat ctccctcttc ctggctctca 661 ccctcctctt gtttcttcga aaactccact gcacgcgcaa ctacatccac atgaacttgt 721 ttgcttcttt catcctgaga accctggctg tactggtgaa ggacgtcgtc ttctacaact 781 cttactccaa gaggcctgac aatgagaatg ggtggatgtc ctacctgtca gagatgtcca 841 cctcctgccg ctcagtccag gttctcttgc attactttgt gggtgccaat tacttatggc 901 tgctggttga aggcctctac ctccacacgc tgctggagcc cacagtgctt cctgagaggc 961 ggctgtggcc cagatacctg ctgttgggtt gggccttccc tgtgctattt gttgtaccct 1021 ggggtttcgc ccgtgcacac ctggagaaca cagggtgctg gacaacaaat gggaataaga 1081 aaatctggtg gatcatccga ggacccatga tgctctgtgt aacagtcaat ttcttcatct 1141 tcctgaaaat tctcaagctt ctcatttcta agctcaaagc tcatcaaatg tgcttcagag 1201 attataaata cagattggca aaatcaacac tggtcctcat tcctttattg ggcgttcatg 1261 agatccTCTT CTCTTTCATC ACTGATGATC AAGTTGAAGG ATTTGCAAAA CTTATACGAC 1321 TTTTCATTCA GTTGACACTG AGCTCCTTTC ATGGGTTCCT GGTGGCCTTG CAGTATGGTT 1381 TTGCCAATGG AGAGGTGAAG GCTGAGCTGC GGAAATACTG GGTCCGCTTC TTGCTAGCCC 1441 GCCACTCAGG CTGCAGAGCC TGTGTCCTGG GGAAGAACTT CCGGTTCCTA GGAAAATGTC 1501 CCAAGAAGCT CTCGGAAGGA GATGGCGCTG AGAAGCTTCG GAAGCTGCAG CCCTCACTTA 1561 ACAGTGGGCG GCTCCTACAC CTAGCCATGC GAGGTCTTGG GGAGCTGGGC GCCCAGCCCC 1621 AACAGGACCA TGCACGCTGG CCCCGGGGCA GCAGCCTGTC CGAGTGCAGT GAGGGGGATG 1681 TCACCATGGC CAACACCATG GAGGAGATTC TGGAAGAGAG TGAGATCTTG CCAACTTTCT 1741 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1801 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1861 GTGGAAAGGA CGAAGTCGGT GAACTTCCGA TAGCCGTACG CGTTAAGTCg acaatcaacc 1921 tctggattac aaaatttgtg aaagatt