Construct: ORF TRCN0000468514
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006239.1_s317c1
- Derived from:
- ccsbBroadEn_10722
- DNA Barcode:
- CATTGAAAAGGAATCTTACTCCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SEPTIN7 (989)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468514
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 989 | SEPTIN7 | septin 7 | XM_017012857.1 | 96% | 95.1% | (many diffs) |
| 2 | human | 989 | SEPTIN7 | septin 7 | NM_001788.5 | 95.6% | 95.6% | 1_57del |
| 3 | human | 989 | SEPTIN7 | septin 7 | NM_001011553.3 | 95.4% | 95.4% | 1_57del;60_61insGTA |
| 4 | human | 989 | SEPTIN7 | septin 7 | XM_024447010.1 | 95.4% | 95.4% | 1_57del;60_61insGTA |
| 5 | human | 989 | SEPTIN7 | septin 7 | XM_017012856.1 | 95.1% | 95.1% | 1_57del;66_67insCAACAG |
| 6 | human | 989 | SEPTIN7 | septin 7 | XM_017012855.1 | 93.7% | 92.8% | (many diffs) |
| 7 | human | 989 | SEPTIN7 | septin 7 | XM_006715806.3 | 93.3% | 93.3% | 1_57del;999_1031del |
| 8 | human | 989 | SEPTIN7 | septin 7 | XM_017012858.2 | 93.1% | 90.8% | (many diffs) |
| 9 | human | 989 | SEPTIN7 | septin 7 | XM_017012859.1 | 93.1% | 90.8% | (many diffs) |
| 10 | human | 989 | SEPTIN7 | septin 7 | XM_017012860.1 | 93.1% | 90.8% | (many diffs) |
| 11 | human | 989 | SEPTIN7 | septin 7 | XM_017012861.1 | 93.1% | 90.8% | (many diffs) |
| 12 | human | 989 | SEPTIN7 | septin 7 | NM_001242956.1 | 92.3% | 91.4% | (many diffs) |
| 13 | human | 989 | SEPTIN7 | septin 7 | NM_001363715.2 | 91.8% | 91.8% | 0_1ins102 |
| 14 | human | 989 | SEPTIN7 | septin 7 | XM_017012862.1 | 91.8% | 91.8% | 0_1ins102 |
| 15 | human | 989 | SEPTIN7 | septin 7 | XM_011515661.2 | 82.6% | 81.7% | (many diffs) |
| 16 | human | 989 | SEPTIN7 | septin 7 | XM_011515656.2 | 82.2% | 75.3% | (many diffs) |
| 17 | human | 641977 | SEPTIN7P2 | septin 7 pseudogene 2 | NR_024271.1 | 46.4% | (many diffs) | |
| 18 | mouse | 235072 | Sept7 | septin 7 | NM_009859.4 | 90.9% | 95.1% | (many diffs) |
| 19 | mouse | 235072 | Sept7 | septin 7 | XM_006510190.3 | 90.7% | 94.7% | (many diffs) |
| 20 | mouse | 235072 | Sept7 | septin 7 | NM_001205367.1 | 90.6% | 94.9% | (many diffs) |
| 21 | mouse | 235072 | Sept7 | septin 7 | XM_006510191.3 | 87.6% | 91.6% | (many diffs) |
| 22 | mouse | 235072 | Sept7 | septin 7 | XM_006510192.3 | 87.6% | 91.6% | (many diffs) |
| 23 | mouse | 235072 | Sept7 | septin 7 | XM_006510193.3 | 87.6% | 91.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1323
- ORF length:
- 1254
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggtagctcaa cagaagaacc ttgaaggcta tgtgggattt gccaatctcc 121 caaatcaagt atacagaaaa tcggtgaaga gaggttttga attcacgctt atggtagtgg 181 gtgaatctgg attgggaaag tcgacattaa tcaactcatt attcctcaca gatttgtatt 241 ctccagagta tccaggtcct tctcatagaa ttaaaaagac tgtacaggtg gaacaatcca 301 aagttttaat caaagaaggt ggtgttcagt tgctgctcac aatagttgat accccaggat 361 ttggagatgc agtggataat agtaattgct ggcagcctgt tatcgactac attgatagta 421 aatttgagga ctacctaaat gcagaatcac gagtgaacag acgtcagatg cctgataaca 481 gggtgcagtg ttgtttatac ttcattgctc cttcaggaca tggacttaaa ccattggata 541 ttgagtttat gaagcgtttg catgaaaaag tgaatatcat cccacttatt gccaaagcag 601 acacactcac accagaggaa tgccaacagt ttaaaaaaca gataatgaaa gaaatccaag 661 aacataaaat taaaatatac gaatttccag aaacagatga tgaagaagaa aataaacttg 721 ttaaaaagat aaaggaccgt ttacctcttg ctgtggtagg tagtaatact atcattgaag 781 ttaatggcaa aagggtcaga ggaaggcagt atccttgggg tgttgctgaa gttgaaaatg 841 gtgaacattg tgattttaca atcctaagaa atatgttgat aagaacacac atgcaggact 901 tgaaagatgt tactaataat gtccactatg agaactacag aagcagaaaa cttgcagctg 961 tgacttataa tggagttgat aacaacaaga ataaagggca gctgactaag agcccTCTGG 1021 CACAAATGGA AGAAGAAAGA AGGGAGCATG TAGCTAAAAT GAAGAAGATG GAGATGGAGA 1081 TGGAGCAGGT GTTTGAGATG AAGGTCAAAG AAAAAGTTCA AAAACTGAAG GACTCTGAAG 1141 CTGAGCTCCA GCGGCGCCAT GAGCAAATGA AAAAGAATTT GGAAGCACAG CACAAAGAAT 1201 TGGAGGAAAA ACGTCGTCAG TTCGAGGATG AGAAAGCAAA CTGGGAAGCT CAACAACGTA 1261 TTTTAGAACA ACAGAACTCT TCAAGAACCT TGGAAAAGAA CAAGAAGAAA GGGAAGATCT 1321 TTTTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1381 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1441 GGCTTTATAT ATCTTGTGGA AAGGACGACA TTGAAAAGGA ATCTTACTCC CCACGCGTTA 1501 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt