Construct: ORF TRCN0000468515
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006685.1_s317c1
- Derived from:
- ccsbBroadEn_00749
- DNA Barcode:
- AGTTTGGAGAATACTCCATGGCAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HLX (3142)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468515
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3142 | HLX | H2.0 like homeobox | NM_021958.4 | 100% | 100% | |
| 2 | mouse | 15284 | Hlx | H2.0-like homeobox | NM_008250.2 | 83.2% | 86.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1530
- ORF length:
- 1464
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt cgcagccggg ctggctccct tctacgcctc caacttcagc ctctggtcgg 121 ccgcttactg ctcctcggcc ggcccaggcg gctgctcctt ccccttggac cccgccgccg 181 tcaaaaagcc ctccttctgc atcgcagaca ttctgcacgc cggcgtgggg gatctggggg 241 cggccccgga gggcctggca ggggcctcgg ccgccgccct caccgcgcac ttgggctcgg 301 ttcacccgca cgcctctttc caagcggcgg ccagatcccc gcttcgaccc accccagtgg 361 tggcgccctc cgaagtcccg gctggcttcc cgcagcggct gtctccgctc tcagccgcct 421 accaccacca tcacccgcaa caacaacagc agcagcaaca gccgcagcag caacagcctc 481 cgcctccgcc ccgggctggc gccctgcagc ccccggcctc ggggacgcga gtggttccga 541 acccccacca cagtggctct gccccggccc cctccagcaa agacctcaaa tttggaattg 601 accgcatttt atctgcagaa tttgacccaa aagtcaaaga aggcaacacg ctgagagatc 661 tcacttccct gctaaccggt gggcggcccg ccggggtgca cctctcaggc ctgcagccct 721 cggccggcca gttcttcgca tctctagatc ccattaacga ggcttctgca atcctgagtc 781 ccttaaactc gaacccaaga aattcagttc agcatcagtt ccaagacacg tttccaggtc 841 cctatgctgt gctcacgaag gacaccatgc cgcagacgta caaaaggaag cgttcatggt 901 cgcgcgctgt gttctccaac ctgcagagga aaggcctgga gaaaaggttt gagattcaga 961 agtacgtgac caagccggac cgaaagcagc tggcggcgat gctgggcctc acggacgcac 1021 aggtgaaggt gtggttccag aaccggcgga tgaagtggcg gcactccaag gaggcccagg 1081 cccaaaagga caaggacaag gaggctggcg agaagccatc aggtggagcc ccggctgcgg 1141 atggcgagca ggacgagagg agccccagcc gttctgaagg cgaggctgag agcgagagca 1201 gcgacTCCGA GTCCCTGGAC ATGGCCCCCA GCGACACGGA GCGGACTGAG GGGAGTGAGC 1261 GTTCTCTGCA CCAAACAACA GTTATTAAGG CCCCGGTCAC TGGCGCCCTC ATTACCGCCA 1321 GCAGTGCTGG GAGTGGTGGG AGCAGCGGCG GCGGCGGCAA TAGTTTCAGC TTCAGCAGCG 1381 CCAGCAGTCT TAGTAGCAGC AGCACCAGTG CGGGTTGCGC CAGCAGCCTT GGCGGCGGCG 1441 GCGCCTCGGA GCTTCTCCCT GCAACACAGC CCACAGCCAG CAGCGCTCCC AAAAGCCCCG 1501 AGCCAGCCCA AGGCGCGCTT GGCTGCTTAT ACCCAACTTT CTTGTACAAA GTGGTTGATA 1561 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1621 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAAGTTT 1681 GGAGAATACT CCATGGCACA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1741 tgaaagatt