Construct: ORF TRCN0000468516
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001028.1_s317c1
- Derived from:
- ccsbBroadEn_05488
- DNA Barcode:
- TCTTGTAGACCGCTTTCAGGGATG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RD3 (343035)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468516
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 343035 | RD3 | retinal degeneration 3, GUC... | NM_001164688.1 | 100% | 100% | |
| 2 | human | 343035 | RD3 | retinal degeneration 3, GUC... | NM_183059.2 | 100% | 100% | |
| 3 | human | 343035 | RD3 | retinal degeneration 3, GUC... | XM_017001151.1 | 94.2% | 94.2% | 1_36del |
| 4 | mouse | 74023 | Rd3 | retinal degeneration 3 | NM_023727.4 | 83% | 85.6% | (many diffs) |
| 5 | mouse | 74023 | Rd3 | retinal degeneration 3 | XM_006497205.2 | 83% | 85.6% | (many diffs) |
| 6 | mouse | 74023 | Rd3 | retinal degeneration 3 | XM_006497206.3 | 83% | 85.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 651
- ORF length:
- 585
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc tctcatctca tggcttcggt ggaacgaggc cccatcccgg ctgtccacca 121 ggagccctgc tgagatggtg ctggagacgc ttatgatgga gctgacgggg cagatgcgag 181 aggctgagag gcagcagcgg gagcgcagca atgcggtcag aaaggtctgc accggtgtgg 241 actacagctg gctggccagc acaccccggt ccacctatga ccTCAGCCCC ATTGAGCGGT 301 TGCAGCTGGA AGATGTCTGC GTTAAGATCC ACCCATCCTA TTGTGGGCCT GCTATCCTCA 361 GGTTCCGGCA GCTGCTGGCG GAGCAGGAGC CCGAGGTGCA GGAGGTGTCC CAGCTCTTCC 421 GCTCGGTGCT GCAGGAGGTC CTGGAGAGGA TGAAGCAGGA AGAGGAGGCC CACAAGCTGA 481 CGCGCCAGTG GAGCCTGCGG CCCCGCGGCA GCCTGGCCAC CTTCAAGACC CGCGCGCGCA 541 TCTCGCCCTT CGCCAGCGAC ATCAGGACCA TCTCCGAGGA CGTGGAGCGG GACACACCGC 601 CGCCACTGCG GTCCTGGAGC ATGCCCGAAT TCCGGGCGCC CAAAGCCGAC TGCCCAACTT 661 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 721 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 781 CTTGTGGAAA GGACGATCTT GTAGACCGCT TTCAGGGATG ACGCGTTAAG TCgacaatca 841 acctctggat tacaaaattt gtgaaagatt