Transcript: Human NM_183059.2

Homo sapiens retinal degeneration 3, GUCY2D regulator (RD3), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-09-22
Taxon:
Homo sapiens (human)
Gene:
RD3 (343035)
Length:
4290
CDS:
1165..1752

Additional Resources:

NCBI RefSeq record:
NM_183059.2
NBCI Gene record:
RD3 (343035)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_183059.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000424655 TATCCTCAGGTTCCGGCAGCT pLKO_005 1452 CDS 100% 0.720 0.576 N RD3 n/a
2 TRCN0000116068 GCTGGAAGATGTCTGCGTTAA pLKO.1 1404 CDS 100% 10.800 7.560 N RD3 n/a
3 TRCN0000116067 CCTGCATGAATAGTGTCTGTT pLKO.1 2391 3UTR 100% 4.950 3.465 N RD3 n/a
4 TRCN0000116069 GTTAAGATCCACCCATCCTAT pLKO.1 1420 CDS 100% 4.950 3.465 N RD3 n/a
5 TRCN0000116071 AGACGCTTATGATGGAGCTGA pLKO.1 1244 CDS 100% 2.640 1.848 N RD3 n/a
6 TRCN0000116070 CGCCAGCGACATCAGGACCAT pLKO.1 1650 CDS 100% 0.000 0.000 N RD3 n/a
7 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 3571 3UTR 100% 5.625 2.813 Y KLHL30 n/a
8 TRCN0000166364 CACACACACACACACACACAA pLKO.1 827 5UTR 100% 4.950 2.475 Y KAAG1 n/a
9 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 3571 3UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_183059.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_05488 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_05488 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000468516 TCTTGTAGACCGCTTTCAGGGATG pLX_317 62.6% 100% 100% V5 n/a
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