Construct: ORF TRCN0000468519
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010905.1_s317c1
- Derived from:
- ccsbBroadEn_10854
- DNA Barcode:
- CATCCACAGGTATCCAATTATTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GOLGA4 (2803)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468519
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2803 | GOLGA4 | golgin A4 | XM_017006188.1 | 7.5% | 7.2% | (many diffs) |
| 2 | human | 2803 | GOLGA4 | golgin A4 | XM_005265075.1 | 7.3% | 7.2% | (many diffs) |
| 3 | human | 2803 | GOLGA4 | golgin A4 | NM_002078.5 | 7.3% | 7.1% | (many diffs) |
| 4 | human | 2803 | GOLGA4 | golgin A4 | NM_001172713.2 | 7.3% | 7.1% | (many diffs) |
| 5 | human | 2803 | GOLGA4 | golgin A4 | XM_005265074.1 | 7.3% | 7.1% | (many diffs) |
| 6 | human | 2803 | GOLGA4 | golgin A4 | XM_006713110.2 | 7.3% | 7% | (many diffs) |
| 7 | human | 2803 | GOLGA4 | golgin A4 | XM_017006187.1 | 7.3% | 7.2% | (many diffs) |
| 8 | human | 2803 | GOLGA4 | golgin A4 | XM_005265073.1 | 7.3% | 7.1% | (many diffs) |
| 9 | human | 2803 | GOLGA4 | golgin A4 | XM_017006184.1 | 7.2% | 7% | (many diffs) |
| 10 | human | 2803 | GOLGA4 | golgin A4 | XM_005265071.3 | 7.2% | 7% | (many diffs) |
| 11 | human | 2803 | GOLGA4 | golgin A4 | XM_005265070.3 | 7.2% | 7% | (many diffs) |
| 12 | human | 2803 | GOLGA4 | golgin A4 | XM_005265072.3 | 7.2% | 7% | (many diffs) |
| 13 | human | 2803 | GOLGA4 | golgin A4 | XM_005265069.3 | 7.2% | 7% | (many diffs) |
| 14 | human | 2803 | GOLGA4 | golgin A4 | XM_017006185.1 | 5.7% | 5.5% | (many diffs) |
| 15 | human | 2803 | GOLGA4 | golgin A4 | XM_017006186.1 | 5.7% | 5.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 564
- ORF length:
- 498
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt caagaaactg aagcaaaaga tcagcgagga gcagcagcag ctccagcagg 121 cgctggctcc tgctcaggcg tcctccaatt cttcaacacc aacaagaatg aggagcagga 181 catcttcatt tacagagcaa cttgatgaag gtacaccCAA TAGAGAGTCA GGTGACACAC 241 AGTCTTTTGC ACAGAAGCTC CAGCTCCGGG TGCCCTCCGT GGAGTCTTTG TTTCGAAGTC 301 CGATAAAGGA ATCTCTATTC CGGTCTTCTT CTAAAGAGTC TTTGGTACGA ACATCTTCCA 361 GAGAATCCCT GAATCGACTT GACCTGGACA GTTCTACTGC CAGTTTTGAT CCACCCTCTG 421 ATATGGATAG CGAGGCTGAA GACTTGGTAG GGAATTCAGA CAGTCTCAAC AAAGAACAGT 481 TGATTCAGCG GTTGCGAAGA ATGGAACGAA GCTTAAGTAG CTACAGGGGA AAATATTCTG 541 AGTCATGGCT CCGATCTTCA TCTTGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 601 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 661 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC ATCCACAGGT 721 ATCCAATTAT TTTACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 781 att