Construct: ORF TRCN0000468522
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008905.1_s317c1
- Derived from:
- ccsbBroadEn_01885
- DNA Barcode:
- CCTTTCGCCTAAACGATCGCGTCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KLRC4 (8302)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468522
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8302 | KLRC4 | killer cell lectin like rec... | NM_013431.2 | 100% | 100% | |
| 2 | human | 3822 | KLRC2 | killer cell lectin like rec... | NM_002260.3 | 61.9% | 52.4% | (many diffs) |
| 3 | human | 3823 | KLRC3 | killer cell lectin like rec... | NM_002261.2 | 59.2% | 52.6% | (many diffs) |
| 4 | human | 3823 | KLRC3 | killer cell lectin like rec... | NM_007333.2 | 55.4% | 49.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 540
- ORF length:
- 474
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa taaacaaaga ggaacctact cagaagtgag tctggcccag gacccaaaga 121 ggcagcaaag gaaacttaag ggcaataaaa tctccatttc aggaaccaaa caggaaatat 181 tccaagtaga attaaacctT CAAAATGCTT CTTCGGATCA TCAAGGGAAT GACAAGACAT 241 ATCACTGCAA AGGTTTACTG CCACCTCCAG AGAAGCTCAC TGCTGAGGTC CTAGGAATCA 301 TTTGCATTGT CCTGATGGCC ACTGTGTTAA AAACAATAGT TCTTATTCCT TGTATTGGAG 361 TACTGGAGCA GAACAATTTT TCCCTGAATA GAAGAATGCA GAAAGCACGT CATTGTGGCC 421 ATTGTCCTGA GGAGTGGATT ACATATTCCA ACAGTTGTTA TTACATTGGT AAGGAAAGAA 481 GAACTTGGGA AGAAAGAGTT TGCTGGCCTG TGCTTCGAAG AACTCTGATC TGCTTTCTAT 541 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 601 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 661 TTTATATATC TTGTGGAAAG GACGACCTTT CGCCTAAACG ATCGCGTCTA CGCGTTAAGT 721 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt