Construct: ORF TRCN0000468534
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016101.1_s317c1
- Derived from:
- ccsbBroadEn_06534
- DNA Barcode:
- TTACGATGGTTATGACATTATATG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- LNPEP (4012)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468534
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4012 | LNPEP | leucyl and cystinyl aminope... | NM_175920.4 | 99.9% | 84.9% | 1267A>G;2582C>A |
| 2 | human | 4012 | LNPEP | leucyl and cystinyl aminope... | NM_005575.3 | 98.5% | 83.8% | 1_42del;1309A>G;2624C>A |
| 3 | human | 4012 | LNPEP | leucyl and cystinyl aminope... | XM_024446045.1 | 98.5% | 83.8% | 1_42del;1309A>G;2624C>A |
| 4 | mouse | 240028 | Lnpep | leucyl/cystinyl aminopeptidase | NM_172827.3 | 85.9% | 73.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2649
- ORF length:
- 2580
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gattgaaaac agcatgtttg aggaagaacc agatgtggtg gatttagcca 121 aagagccttg tttacatcct ctagagcctg atgaggtgga atatgagccc cggggttccc 181 gactgctggt gcggggtctt ggtgagcatg agatggagga ggatgaagag gattatgagt 241 catcagcaaa gctgctgggc atgtccttca tgaatagaag ctcaggcctt cggaacagtg 301 caactggtta caggcagagc ccagatgggg cttgttcagt accctctgca aggaccatgg 361 tggtctgtgc ttttgtcatc gtggttgctg tttctgtaat catggtgatt tacttactgc 421 ccagatgtac ctttaccaaa gaaggctgcc ataaaaaaaa ccagtcaatt ggactaattc 481 agccatttgc aacaaatggg aaattgtttc catgggcaca gatcaggctt cccactgccg 541 ttgtgccact acgctatgaa ctcagcctac acccgaacct aacctcgatg acattcaggg 601 gttctgtgac aatttcagtt caggctcttc aggtcacatg gaatatcatt cttcatagca 661 caggtcataa tatttcaaga gtgaccttta tgtcagcagt ttcaagccaa gaaaaacaag 721 ctgagatcct ggaatatgca tatcatggac agatcgccat tgttgccccc gaagcccttc 781 tagcagggca caattatacg ttgaagatag agtactcggc aaatatatct agttcttatt 841 atgggtttta tggcttctcc tacacagatg aaagtaatga gaaaaagtac tttgcagcaa 901 ctcagtttga acccctggca gcaagatctg cttttccttg ttttgatgaa ccagcattta 961 aagccacttt tatcatcaag atcataaggg atgagcaata caccgcttta tcaaatatgc 1021 ctaagaagtc atcagtcgtt ctagatgatg gacttgttca ggatgagttt tctgagagtg 1081 tgaagatgag cacttacttg gttgctttca ttgtgggaga gatgaagaac ctgagtcagg 1141 acgtaaatgg aaccctggtt tctatatatg ctgtaccaga aaagattggt caagttcatt 1201 atgccttgga aacaactgtg aagcttcttg agttttttca aaactacttt gaaattcagt 1261 acccacttaa gaaattggat ttggtggcta ttcctgactt tgaagcagga gcaatggaaa 1321 attggggttt gctcgccttc cgagaggaga cacttctgta tgacagtaac acttcttcaa 1381 tggcggatag aaagctggtg actaaaatca ttgctcatga gctggcccac cagtggtttg 1441 gcaatctggt aacaatgaag tggtggaatg acctatggct aaatgaaggt tttgccactt 1501 tcatggagta tttctctttg gaaaaaatat tcaaagagct ttctagttat gaagatttct 1561 tagatgctcg atttaaaacc atgaagaaag attccttaaa ttcatctcat ccaatatcat 1621 catctgttca gtcttcagaa caaattgaag aaatgtttga ttctctttcc tattttaagg 1681 gatcttctct cttgttgatg ttgaaaactt accttagtga agatgtgttt caacatgctg 1741 ttgtccttta cctgcataat cacagctatg catctattca aagtgatgat ctgtgggata 1801 gttttaatga ggtcacaaac caaacactag atgtaaagag aatgatgaaa acctggaccc 1861 tgcagaaagg atttccttta gtgactgttc aaaagaaagg aaaggaactt tttatacaac 1921 aagagagatt ctttttaaat atgaagcctg aaattcagcc ttcagataca agctacctgt 1981 ggcatattcc actatcctat gtcactgaag gaagaaatta ttcaaaatat caatcggtat 2041 cattactgga taagaaatca ggtgtcatca atcttacaga agaagtgctg tgggtcaaag 2101 tgaatataaa catgaatggt tattatattg tacactatgc agatgatgat tgggaagcac 2161 taatccatca gttgaaaata aatccttatg ttctgagtga caaagaccga gccaacctta 2221 tcaacaacat ctttgaactt gcaggcctag gcaaggtacc tctcaagagg gcctttgatt 2281 tgattaatta tcttggaaat gagaaccata ctgcacccat caccgaagcc ctgtttcaga 2341 cagacctcat ctataacctc cttgaaaaac tgggatacat ggatctggcc tcaagactgg 2401 tgactagggt atttaaatta cttcaaaacc aaattcaaca acaaacttgg actgatgagg 2461 gcactccatc tatgcgagag cttcggtcag ccctgctaga gtttgcttgc acccacaacc 2521 tggggaactg ctctactact gccatgaaac tgtttgatga ctggatggca tccaatggaa 2581 ctcaaagcct acctactgat gtcatgacaa ctgtgttcaa agttggagca aaaactgaca 2641 aaggctggta attccttttg ggcaaataca tttctatagg ctctgaagca gagaagaaca 2701 aaatactaga agcacttgcc agctcagagg atgtgcggaa gctttactgg ttaatgaaaa 2761 gtagcctgaa tggagataac ttccgaacac agaagctgtc ttttatcatt agaacagtgg 2821 gtcgacattt tcctggacac ttactggcat gggattttgt caaagagaac tggaataagc 2881 ttGTACAGAA GTTCCCTCTG GGGTCCTATA CCATACAAAA TATTGTTGCT GGATCAACTT 2941 ACCTGTTTTC AACAAAGACA CATTTATCTG AGGTTCAGGC ATTCTTTGAA AATCAGTCAG 3001 AGGCAACCTT CCGGCTTCGT TGTGTCCAGG AGGCTTTGGA AGTCATTCAG TTGAATATCC 3061 AGTGGATGGA GAAGAACCTC AAAAGTCTCA CATGGTGGCT GTTGCCAACT TTCTTGTACA 3121 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 3181 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 3241 AGGACGATTA CGATGGTTAT GACATTATAT GACGCGTTAA GTCgacaatc aacctctgga 3301 ttacaaaatt tgtgaaagat t