Construct: ORF TRCN0000468539
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008827.1_s317c1
- Derived from:
- ccsbBroadEn_00304
- DNA Barcode:
- ACAGCACGTGTAGCGCAGTGAGGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CHRM2 (1129)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468539
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1129 | CHRM2 | cholinergic receptor muscar... | NM_000739.2 | 100% | 100% | |
| 2 | human | 1129 | CHRM2 | cholinergic receptor muscar... | NM_001006626.2 | 100% | 100% | |
| 3 | human | 1129 | CHRM2 | cholinergic receptor muscar... | NM_001006627.2 | 100% | 100% | |
| 4 | human | 1129 | CHRM2 | cholinergic receptor muscar... | NM_001006628.2 | 100% | 100% | |
| 5 | human | 1129 | CHRM2 | cholinergic receptor muscar... | NM_001006629.2 | 100% | 100% | |
| 6 | human | 1129 | CHRM2 | cholinergic receptor muscar... | NM_001006630.1 | 100% | 100% | |
| 7 | human | 1129 | CHRM2 | cholinergic receptor muscar... | NM_001006631.2 | 100% | 100% | |
| 8 | human | 1129 | CHRM2 | cholinergic receptor muscar... | NM_001006632.2 | 100% | 100% | |
| 9 | human | 1129 | CHRM2 | cholinergic receptor muscar... | XM_024446648.1 | 100% | 100% | |
| 10 | mouse | 243764 | Chrm2 | cholinergic receptor, musca... | NM_203491.3 | 89.3% | 96.3% | (many diffs) |
| 11 | mouse | 243764 | Chrm2 | cholinergic receptor, musca... | XM_006506136.2 | 89.3% | 96.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1467
- ORF length:
- 1398
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaataactca acaaactcct ctaacaatag cctggctctt acaagtcctt 121 ataagacatt tgaagtggtg tttattgtcc tggtggctgg atccctcagt ttggtgacca 181 ttatcgggaa catcctagtc atggtttcca ttaaagtcaa ccgccacctc cagaccgtca 241 acaattactt tttattcagc ttggcctgtg ctgaccttat cataggtgtt ttctccatga 301 acttgtacac cctctacact gtgattggtt actggccttt gggacctgtg gtgtgtgacc 361 tttggctagc cctggactat gtggtcagca atgcctcagt tatgaatctg ctcatcatca 421 gctttgacag gtacttctgt gtcacaaaac ctctgaccta cccagtcaag cggaccacaa 481 aaatggcagg tatgatgatt gcagctgcct gggtcctctc tttcatcctc tgggctccag 541 ccattctctt ctggcagttc attgtagggg tgagaactgt ggaggatggg gagtgctaca 601 ttcagttttt ttccaatgct gctgtcacct ttggtacggc tattgcagcc ttctatttgc 661 cagtgatcat catgactgtg ctatattggc acatatcccg agccagcaag agcaggataa 721 agaaggacaa gaaggagcct gttgccaacc aagaccccgt ttctccaagt ctggtacaag 781 gaaggatagt gaagccaaac aataacaaca tgcccagcag tgacgatggc ctggagcaca 841 acaaaatcca gaatggcaaa gcccccaggg atcctgtgac tgaaaactgt gttcagggag 901 aggagaagga gagctccaat gactccacct cagtcagtgc tgttgcctct aatatgagag 961 atgatgaaat aacccaggat gaaaacacag tttccacttc cctgggccat tccaaagatg 1021 agaactctaa gcaaacatgc atcagaattg gcaccaagac cccaaaaagt gacTCATGTA 1081 CCCCAACTAA TACCACCGTG GAGGTAGTGG GGTCTTCAGG TCAGAATGGA GATGAAAAGC 1141 AGAATATTGT AGCCCGCAAG ATTGTGAAGA TGACTAAGCA GCCTGCAAAA AAGAAGCCTC 1201 CTCCTTCCCG GGAAAAGAAA GTCACCAGGA CAATCTTGGC TATTCTGTTG GCTTTCATCA 1261 TCACTTGGGC CCCATACAAT GTCATGGTGC TCATTAACAC CTTTTGTGCA CCTTGCATCC 1321 CCAACACTGT GTGGACAATT GGTTACTGGC TTTGTTACAT CAACAGCACT ATCAACCCTG 1381 CCTGCTATGC ACTTTGCAAT GCCACCTTCA AGAAGACCTT TAAACACCTT CTCATGTGTC 1441 ATTATAAGAA CATAGGCGCT ACAAGGTTGC CAACTTTCTT GTACAAAGTG GTTGATATCG 1501 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1561 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAACAGCACG 1621 TGTAGCGCAG TGAGGGACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1681 aagatt