Construct: ORF TRCN0000468543
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000926.1_s317c1
- Derived from:
- ccsbBroadEn_04245
- DNA Barcode:
- TTGACTTTCGAGAACTGCCCGCTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LBH (81606)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468543
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 81606 | LBH | LBH regulator of WNT signal... | NM_030915.4 | 100% | 100% | |
| 2 | human | 81606 | LBH | LBH regulator of WNT signal... | XM_017005059.1 | 42.1% | 42.3% | 1_375del;376_377ins24 |
| 3 | mouse | 77889 | Lbh | limb-bud and heart | NM_029999.4 | 89.5% | 90.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 384
- ORF length:
- 315
- Sequence:
-
1 TCTTCCATTT CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCACCAT GTCTATATAT TTCCCCATTC ACTGCCCCGA CTATCTGAGA TCGGCCAAGA 121 TGACTGAGGT GATGATGAAC ACCCAGCCCA TGGAGGAGAT CGGCCTCAGC CCCCGCAAGG 181 ATGGCCTTTC CTACCAGATC TTCCCAGACC CGTCAGATTT TGACCGCTGC TGCAAACTGA 241 AGGACCGTCT GCCCTCCATA GTGGTGGAAC CCACAGAAGG GGAGGTGGAG AGCGGGGAGC 301 TCCGGTGGCC CCCTGAGGAG TTCCTGGTCC AGGAGGATGA GCAAGATAAC TGCGAAGAGA 361 CAGCGAAAGA AAATAAAGAG CAGTTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 421 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 481 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT TGACTTTCGA 541 GAACTGCCCG CTAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 601 att